Solid fermentation production method of bacillus coagulans

A technology of bacillus coagulans and solid fermentation, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of failure to meet the requirements of production and use, complicated process, difficult and simple operation, etc., and achieve easy Production and promotion, high density of living bacteria, simple and stable production process

Inactive Publication Date: 2013-09-11
GUANGZHOU GLAM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for efficient growth of bacilli that are resistant against harmful microorganisms such as Salmonella or Escherichia coli (ECO). It also makes them easier to make into products like foods with improved nutritional value by adding these substances during processing steps.

Problems solved by technology

This patented technical problem addressed in this patent relates to improving the health benefits associated with certain types of yeast called Saccharomyces crustacearicus, while reducing their negative impact upon human gut cells like gastrointestinals. Previous researchers found ways to protect these organisms during storage without affecting them when they are ingested. However, previous methods were complex due to poor efficiency and safety concerns.

Method used

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  • Solid fermentation production method of bacillus coagulans
  • Solid fermentation production method of bacillus coagulans
  • Solid fermentation production method of bacillus coagulans

Examples

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Effect test

Embodiment 1

[0024] Example 1 The technical process of producing Bacillus coagulans culture by solid fermentation, the steps are as follows:

[0025] (1) Strain activation: the present invention adopts Bacillus coagulans ( Bacillus coagulans ) as a fermentation strain, the preservation number: GIM 1.421, was purchased from Guangdong Microbial Culture Collection Center. Put the Bacillus coagulans strains stored at -80℃ in the seed medium (tryptone 10g, yeast extract 5g, NaCl 10g, 25% concentration of MnSO 4 Add to 0.2% and 15g of agar powder, sterilized by high temperature and high pressure for later use) Streak on the solid plate, incubate at 37~40°C for 20~24 hours;

[0026] (2) Preparation of primary seeds: Pick three to five loops of activated Bacillus coagulans and inoculate them in 200 mL liquid seed medium with seed medium (tryptone 10g, yeast extract 5g, NaCl 10g and 25% concentration MnSO 4 Add to 0.2%, after high temperature and high pressure sterilization, put the triangular f...

Embodiment 2

[0030] Example 2 Acute Toxicity Test in Mice Produced by Solid Fermentation of Bacillus coagulans Culture

[0031] Select 40 healthy NIH mice, male or female, weighing about 30 g, and randomly divide them into two groups, namely blank control group and Bacillus coagulans culture dry powder group, with 20 mice in each group. Before the experiment, the weight of the mice was recorded once. At the beginning of the experiment, the mice in each group were given the test sample at a time with a maximum administration volume of 0.8ml. After the administration, the mice were continuously observed for 7 days to observe whether the mice appeared mentally or not within 7 days. , activities, abnormal diet and death. For the dead mice, the heart, liver, spleen, lung, kidney, etc. should be dissected immediately, and the pathological changes should be recorded. On the 8th day, the body weight of the mice in each group was recorded, and the differences among the groups were compared; then a...

Embodiment 3

[0038] Example 3 Application of Bacillus coagulans feed products to promote the growth of weaned piglets

[0039] In the experiment, 320 weaned piglets (285 binary piglets and 36 ternary piglets) were randomly divided into control group and experimental group, with 160 piglets in each group and 160 repetitions. Bacillus coagulans was not added to the basal diet of the control group, and 1.5% of the culture of Bacillus coagulans was added to the basal diet of the experimental group. There was no significant difference between the initial average weight of the control group and the initial average weight of the experimental group. At the end of the experiment, compared with the control group, the nursery pigs in the experimental group increased by 1.84 kg on average, the average daily gain increased by 43 g, the death rate decreased by 3%, and the feed-to-meat ratio decreased by 0.18. Experiments have proved that Bacillus coagulans feed products can effectively improve the prod...

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Abstract

The invention discloses a solid fermentation production method of bacillus coagulans. The method comprises the steps of: using bacillus coagulans as fermentation strain; culturing the bacillus coagulans in an optimized solid culture medium at 37-40 DEG C; performing large-scale fermentation production by three-stage amplification; performing colony counting on the bacillus coagulans culture, wherein the concentration of viable bacteria is as high as 9.10*10<9>CFU/g fresh yeast, and the concentration of spore is as high as 2.45*10<9>CFU/g fresh yeast. The method disclosed by the invention has the beneficial effects that the production technique is simple and stable; the viable bacteria has high density; the fermentation products can be directly used without separation and purification, and are easy to produce and popularize; meanwhile, bacillus coagulans is added into feed as a feed additive and can be planted in animal caecum, colon and rectum so as to produce a large quantity of anti-microbial coagulin, lactic acid, amino acid and vitamins and various digestive enzymes by fermentation, so that the quality of the feed is improved, digestion and adsorption of the feed are promoted, and the feed conversion ratio is lowered.

Description

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Claims

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Application Information

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Owner GUANGZHOU GLAM BIOTECH
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