Compound microbial preparation and preparation method thereof

A technology of compound microbial inoculum and Beauveria bassiana, applied in the field of environmental protection and microorganisms, can solve the problems of single type of inoculum, poor functionality, low economic benefits, etc. Effect

Active Publication Date: 2013-10-02
攀枝花市绿态美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The technical problem to be solved by the present invention is how to provide a compound microbial bacterial agent that can deodorize and prevent insects, so as to solve the shortcomings of the existing microbial agents that have single types, poor functionality, and low economic benefits

Method used

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  • Compound microbial preparation and preparation method thereof
  • Compound microbial preparation and preparation method thereof
  • Compound microbial preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0075] The preparation method of the composite microbial bacterial agent is as follows: firstly prepare the fermentation broths of EM bacteria, Bacillus subtilis, Beauveria bassiana, and Bacillus thuringiensis respectively; ~8 parts, 3 parts of Bacillus subtilis fermentation broth, 1 part of Beauveria bassiana fermentation broth, and 1 part of Bacillus thuringiensis.

[0076] The preparation method of EM bacterium fermented liquid is:

[0077] Use 10 grams of EM strains, 0.1 kg of brown sugar (the brown sugar is first dissolved in hot water), and add 1 kg of water. Note: (Heat the water to 100°C first, then add red pond or white sugar, and then continue heating for 5 minutes. Add EM strains when it cools to 40°C), and ferment in a sealed container (35-37°C) for 3-5 days, on the third day It can deflate once a day, open the mouth of the container and smell a sweet and sour smell, that is, the activation is successful, and it is used as a liquid strain.

[0078] Add the activa...

experiment example 1

[0090] Experimental example 1 Indoor Garbage Deodorization Test

[0091] Put a pile of highly decomposed and foul-smelling garbage in a sealed room, dilute the prepared compound microbial agent 20 times with tap water before use, and spray it directly on the garbage dump area. Set up different collection locations around the garbage for sampling, measure the concentration of hydrogen sulfide and ammonia in the odorous gas before spraying and 15 minutes after spraying, the sampling point is 1 meter away from the edge of the odor source, the sampling height is 1.4 meters, 6 points are sampled, and the average is taken value. Odor concentration adopts three-point comparative smelly bag method (GB / T14675); ammonia adopts sodium hypochlorite-salicylic acid spectrophotometry method (GB / T14679); hydrogen sulfide adopts polyvinyl alcohol ammonium phosphate absorption-methylene blue colorimetric method (GB / T14679) T16489); the total number of bacteria adopts the impact method (GB / T...

experiment example 2

[0097] 1. Preparation of bacterial agents for testing.

[0098] Obtain the bacterial liquid according to the aforementioned strain culture method, and mix various bacterial liquids in proportion: 5 parts of EM bacterial liquid, 3 parts of Bacillus subtilis liquid, 1 part of Beauveria bassiana liquid, and 1 part of Bacillus thuringiensis liquid, and put them into test tubes .

[0099] 2. The pests used in the test are: locusts, pine caterpillars, cypress aphids, cutworms, inchworms, flies, long beetles, and aphids.

[0100] 3. Test method.

[0101] Take 200 mL of the mixed bacterial solution for the test, pack 80 pests into vials, seal them with gauze, place them in a room temperature room below 25°C, and then spray them with different dilutions into the bottles containing the insects. 48-hour observation and inspection and calculation of pest mortality. The method for identifying death is: add 1-5 drops of 5% NaCl solution to the test vial, observe the dead insects stiffen ...

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Abstract

The invention discloses a compound microbial preparation and a preparation method thereof, and belongs to the fields of environmental protection and microbiology. The compound microbial preparation comprises EM bacteria, bacillus subtilis, beauveria bassiana and bacillus thuringiensis. According to the invention, the compound microbial preparation is prepared from complementary and antergic microbial strains. The compound microbial preparation has advantages of various kinds, reasonable compatibility, strong function, high economic benefit, and the like. The compound microbial preparation has good effects on biological deodorization and plant insect control.

Description

technical field [0001] The invention belongs to the fields of environmental protection and microorganisms, and in particular relates to a composite microbial bacterial agent and a preparation method thereof. Background technique [0002] 1. Odor is a sensory public hazard with many sources and a wide area of ​​pollution. It not only makes people feel unpleasant and disgusting, but also many harmful substances in the odor directly endanger human health. For this reason, since the 1960s, it has gradually attracted the attention and attention of environmental science and technology workers at home and abroad, and has achieved various results. Japan promulgated the "Odor Control Act" in 1971, the United States promulgated the "Clean Air Act" in 1971, and Germany stipulated the frequency and time measurement methods of odor in the "Ordinance on Odor in the Ambient Atmosphere" in 1993 . my country's research on odor pollution started relatively late. With reference to Japan's e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A61L9/01A01N63/04A01P7/04C12R1/125C12R1/07C12R1/645A61L101/52
Inventor 辛玉江
Owner 攀枝花市绿态美生物科技有限公司
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