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Cloning and application of CDC16 gene molecular marker related to pork quality character

A trait, pork technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problem of less CDC16 gene in pigs

Active Publication Date: 2015-05-27
广东食出宝金农业科技有限公司
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  • Claims
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Problems solved by technology

[0008] However, there are very few related studies on porcine CDC16 gene at home and abroad

Method used

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  • Cloning and application of CDC16 gene molecular marker related to pork quality character
  • Cloning and application of CDC16 gene molecular marker related to pork quality character
  • Cloning and application of CDC16 gene molecular marker related to pork quality character

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Embodiment Construction

[0026] The present invention will be further explained below in conjunction with specific examples, but the specific implementation does not limit the present invention in any way.

[0027] The Csp6I polymorphism of CDC16 gene was detected by PCR-RFLP technique.

[0028] Primer design: Using comparative genomics methods, according to the 6th intron to the 8th intron of the human CDC16 gene (GenBank accession number: NW_003541041), use the gene to perform BLAST in GenBank, and screen out CDC16 gene candidates after sequence alignment For the SNPs site, select the 58478bp candidate SNPs site of the CDC16 gene, use the NW_003541041.seq sequence as the standard, design primers, and use PCR-RFLP technology to verify the existence of the site.

[0029] The primers are: F: 5′-GACACGGCAACATGCTAG-3′,

[0030] R: 5′-TTGGGATGGAAGAAGACC-3′

[0031] The DNA samples came from 867 pigs of 8 breeds, including 62 Duroc pigs and 118 Large White pigs from the pig farm of Yiyang Agricultural Sc...

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Abstract

The invention belongs to the technical field of livestock molecular marker preparation, and in particular relates to cloning and application of a porcine CDC16 gene molecular marker. The molecular marker provided by the invention is cloned from a CDC16 gene; and the sequence of the CDC16 molecular marker is shown as a SEQ ID NO:1. An A / G basic group mutation at the No. 570bp in the SEQ ID NO:1 leads to PCR-RFLP-Csp6I polymorphisms, which can be applied to association analysis of pork quality character. The invention provides a novel molecular marker for pork quality character marker assisted selection.

Description

technical field [0001] The invention belongs to the technical field of livestock molecular biology, and relates to a nucleic acid molecule comprising porcine gene nucleotides as shown in SEQ ID NO:1. The present invention also relates to a single nucleotide polymorphism site in the polynucleotide sequence shown in SEQ ID NO: 1 and a method for detecting the single nucleotide polymorphism site. Background technique [0002] Pork is the main source of animal protein for urban and rural residents in my country. As people's demand for meat increases, their requirements for meat quality also increase relatively. The development of molecular biotechnology enables people to search for major genes controlling meat quality traits or molecular markers closely linked to them at the DNA level, which can be applied to marker-assisted selection in the breeding process to improve the selection process and better pork quality traits. Meet people's needs to obtain greater economic benefits....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/12C12N15/11C12Q1/68
Inventor 马海明许栋贺长青肖定福何俊蒋隽王玲玉杨虎
Owner 广东食出宝金农业科技有限公司
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