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Methods of capturing bindable targets from liquids

A bonding and target technology, applied in the direction of biochemical equipment and methods, microbiological determination/inspection, measuring devices, etc., can solve the problems of quantitative difficulty and prolongation

Inactive Publication Date: 2013-10-16
MATRIX MICROSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the need for an enrichment period means that the handling process is usually extended by about 2 days and makes quantification difficult

Method used

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  • Methods of capturing bindable targets from liquids
  • Methods of capturing bindable targets from liquids
  • Methods of capturing bindable targets from liquids

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0060] 10 ml samples of buffered peptone water (BPW) were cultured in duplicate with known levels of Salmonella Salford, Listeria monocytogenes species or E. coli O157.

[0061] One sample from each duplicate was placed in AUTO sample container and pass a standard dose (50 μl) of antibody-coated particles appropriate to the organism for a standard 15 min AUTO capture to analyze.

[0062] The remaining sample was placed in a 15ml conical tube and the same dose of paramagnetic particles (<1 μm diameter) coated with anti-(a) Salmonella, (b) coliform Antibodies to Bacillus O157:H7 or (c) Listeria.

[0063] Particles in the tube are taken out of suspension and collected along the side of the tube using a series of magnets arranged in a vertical line against the wall of the tube. The sample tube is then manually moved away from the set of magnets, and the other set is placed directly on the opposite side of the tube, thereby moving the particles through the sample.

[0064] Th...

example 2

[0076] 10 ml samples of buffered peptone water (BPW) were cultured in duplicate with known levels of Salmonella Tranaroa or E. coli O157.

[0077] One sample from each duplicate was placed in AUTO sample container, and pass a standard dose (50 μl) of antibody-coated pellet suitable for the organism using the standard 15 min AUTO capture to analyze.

[0078] The remaining sample was placed in a 15ml conical tube and the same dose of paramagnetic particles (<1 μm diameter) coated with anti-(a) Salmonella or (b) coliform bacteria was added directly to the sample. Antibodies to Bacillus O157:H7. The pellets in the tube were removed from the suspension and collected along the side of the tube using a series of magnets arranged in a vertical line against the wall of the tube. The sample tube was then manually moved away from the set of magnets, and the other set was placed directly on the opposite side of the tube, causing the pellet to move through the sample.

[0079] The tu...

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Abstract

A bindable target such as a bacterial cell, virus, or molecule is captured from a liquid by contacting the liquid with magnetically attractable particles have an affinity for the target, and causing said particles to move repeatedly through said liquid to at least one solid support zone by attractive magnetic forces to capture the target onto said particles. The particles may be ferromagnetic, paramagnetic or superparamagnetic particles and may bear antibody, antibody binding fragments, a substance having an epitope capable of reacting in a specific manner with an antibody, an aptamer, a nucleic acid sequence or a nucleic acid analogue sequence, biotin, avidin or streptavidin. The particles may be moved back and forth in the liquid between separated solid support zones by attractive magnetic forces which attract the particles temporarily to different solid support zones in turn.

Description

technical field [0001] The present invention relates to a method of capturing a bindable target from a liquid containing said bindable target and to an assay process involving said bindable target. Background technique [0002] Notwithstanding the broad and general applicability of the invention, it is particularly relevant to the problem of monitoring microorganisms in food and water and will be described with particular reference to the context. [0003] Common methods for assaying for levels of organisms such as Cryptosporidium and Salmonella in water and food would be time consuming and labor intensive. [0004] A major problem in the processing of these assays is that the organisms may be present in very small numbers in large volumes of liquid and must first be concentrated in a volume in which they can be detected. [0005] Typically, a water sample is concentrated by passing a large volume of water through a cellulose filter material, which is broken up and placed i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCC12Q1/24G01N33/54333
Inventor A·帕顿
Owner MATRIX MICROSCI
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