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Rhododendron bachii Levl tissue culturing and rapid propagation method

A technology of tissue culture and horse silver flower, which is applied in the field of plant tissue culture, can solve the problems of short subculture cycle, etc., and achieve the effect of fast rooting and survival, high multiplication coefficient, reliable and effective propagation method

Active Publication Date: 2013-10-23
HANGZHOU BOTANICAL GARDEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention aims at the botanical characteristics of P. adenocalyx, and uses the stem section of the flowered mother plant as an explant to establish an efficient tissue culture and rapid propagation system. The tissue culture seedlings grown by it grow robustly and have a short succession cycle, which has not been seen in China. There is a report on the successful tissue culture of P. adenocalyx

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The rapid propagation method of Rhizoma adenatifera tissue culture, the method is carried out according to the following steps:

[0022] 1. Preparation of culture medium

[0023] (1) Induction medium: WPM basic medium + ZT 1.0 mg / L + NAA0.05 mg / L + 20 g / L sucrose + 6 g / L agar, pH4.8, high temperature sterilization;

[0024] (2) Proliferation medium: WPM basic medium + ZT 1.0mg / L + NAA 0.05mg / L + 20g / L sucrose + 6 g / L agar, pH 4.8, high temperature sterilization;

[0025] (3) Strong seedling medium: WPM basic medium + ZT 0.5 mg / L + NAA0.05 mg / L + 20g / L sucrose + 6 g / L agar, pH 4.8, high temperature sterilization;

[0026] 2. Rapid Propagation Method of Tissue Culture of P.

[0027] (1) Selection of explants: In spring, new branches with latent buds are cut from the mother plant of Calyx adenocarpa;

[0028] (2) Sterilization of explants: cut off the branches and leaves, first soak and rinse with tap water for 10 minutes, wash with detergent twice and blot dry; then, d...

Embodiment 2

[0034] The rapid propagation method of Rhizoma adenatifera tissue culture, the method is carried out according to the following steps:

[0035] 1. Preparation of culture medium

[0036] (1) Induction medium: Anderson basic medium + ZT 2.0 mg / L + NAA0.1 mg / L + 20 g / L sugar + 10 g / L agar, pH5.2, high temperature sterilization;

[0037] (2) Proliferation medium: Anderson basic medium + ZT 2.0 mg / L + NAA0.1mg / L + 20g / L sugar + 10 g / L agar, pH 5.2, high temperature sterilization;

[0038] (3) Strong seedling medium: Anderson basic medium + ZT 1.0 mg / L + NAA0.1 mg / L + 20g / L white sugar + 10 g / L agar, pH 5.2, high temperature sterilization;

[0039] 2. Rapid Propagation Method of Tissue Culture of P.

[0040] (1) Selection of explants: In spring, new branches with latent buds are cut from the mother plant of Calyx adenocarpa;

[0041](2) Sterilization of explants: Cut off the branches and leaves, first soak and rinse with tap water for 20 minutes, wash with detergent once and blot...

Embodiment 3

[0047] In this example, the induction medium is: WPM basic medium + ZT 2.0mg / L + NAA 0.1 mg / L + 20 g / L white sugar + 8 g / L agar, pH 5.0, high temperature sterilization; Cut the young shoots from the mother plant of Rhizoma adenocalyx, cut off the shoot tip after sterilization, and insert the remaining shoots obliquely into the bud induction medium at 26°C for 3 weeks in light (16 hours) / black (8 hours) ; Remove the top of the newly grown axillary buds and insert them into the proliferation medium: WPM basic medium + ZT 2.0 mg / L + NAA 0.1 mg / L + 20g / L sugar + 8 g / L agar, pH 5.0, high temperature sterilization , 26°C light (16 hours) / black (8 hours) culture for 6 weeks; cut the clustered shoots that grow to 1-2cm long and transfer them to the strong seedling medium: WPM basic medium + ZT 1.0 mg / L + NAA 0.1 mg / L + 20g / L sugar + 8 g / L agar, pH 5.0, high-temperature sterilization, cultured at 26°C in light (16 hours) / dark (8 hours) for 2 weeks; in January-February every year, it wi...

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Abstract

The invention relates to a Rhododendron bachii Levl tissue culturing and rapid propagation method. The method comprises the steps of explant selection, explant disinfection, axillary bud induced culture, enrichment culture, strong seedling culture and ex vitro rooting. The method treating an annual blossom immature stem segment as the explant and WPM or Anderson as a basal culture medium and utilizing a ZT hormone has the advantages of good induction effect, high propagation coefficient, good cultivated seedlings, and fast rooting and survival, and allows an effective Rhododendron bachii Levl tissue culturing and rapid propagation system to be successfully established. In the invention, the propagation coefficient within a propagation subculture period (6-8 weeks) is controlled in a range of 5-6 times, and the propagation coefficient within one year is about 10000 times, so the propagation coefficient of Rhododendron bachii Levl is greatly improved; and the method is a reliable and effective propagation method for the preservation and sustainable development of the above species and provides a scientific technological support for the landscaping of wild rhododendrons.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a rapid propagation method of Rhododendron calyx in Rhododendronaceae. Background technique [0002] Glandularia calyx ( Rhododendron bachii Levl), Rhododendron genus Rhododendron subgenus Rhododendron Rhododendron, also known as Rhododendron shibi, is an endemic species in China, an evergreen shrub, naturally distributed in Anhui, Zhejiang, Jiangxi, Hubei, Hunan, Guangdong, Guangxi, Sichuan and Guizhou. The wild species has luxuriant flowers and leaves, is easy to introduce and domesticate, has strong adaptability, and has great potential in landscaping. [0003] There is no research on the propagation method of E. adenocarpa, and the method of seed propagation or cutting is generally adopted. In the same subgenus and same group, the propagation method mainly relies on seed propagation, but the plants produced by the seed propagation method need many years to flo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 朱春艳余金良朱丹华吕敏朱剑俊
Owner HANGZHOU BOTANICAL GARDEN
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