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Molecular marker relevant to wheat drought resistance and application thereof

A molecular marker, drought resistance technology, applied in application, microbial determination/inspection, DNA/RNA fragment, etc., can solve the problems of reduced photosynthetic efficiency, harm, enhanced respiration, etc.

Inactive Publication Date: 2014-07-30
NORTHWEST A & F UNIV
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Problems solved by technology

[0003] Adversity stress such as drought will cause a series of changes in physiological and biochemical functions in plants, resulting in enhanced respiration, reduced photosynthetic efficiency, and damage to biofilm structures.

Method used

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  • Molecular marker relevant to wheat drought resistance and application thereof
  • Molecular marker relevant to wheat drought resistance and application thereof
  • Molecular marker relevant to wheat drought resistance and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1. Acquisition and Application of Molecular Markers Related to Wheat Drought Resistance

[0049] 1. Determination of drought resistance of wheat

[0050] Using the 99 wheat varieties (lines) in Table 2 as experimental materials, referring to the "Technical Specifications for Identification and Evaluation of Wheat Drought Resistance (GB / T21127-2007)", the hyperosmotic solution method was used to determine the relative germination of wheat varieties (lines) at the germination stage rate, and carry out the division of drought resistance grades. details as follows:

[0051] 1. Identification of drought resistance during seed germination

[0052] (1) Sample preparation

[0053] After fully mixing the seed samples of the material to be tested, 800 seeds were randomly selected.

[0054] (2) Preparation of stress solution

[0055] Dissolve 192g of polyethylene glycol-6000 (PEG-6000) in 1000mL of deionized water, namely -0.5Mpa PEG-6000 aqueous solution.

[0056] (...

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Abstract

The invention discloses a primer and a molecular marker relevant to wheat drought resistance and application of the primer and the molecular marker. A primer pair for detecting the wheat drought resistance disclosed by the invention is a primer pair A or a primer pair B, wherein the primer pair A is composed of single-chain DNA (Deoxyribonucleic Acid) represented by a sequence 1 in a sequence table, and single-chain DNA represented by a sequence 2 in the sequence table; the primer pair B is composed of single-chain DNA represented by a sequence 3 in the sequence table, and a single-chain DNA represented by a sequence 4 in the sequence table. The primer disclosed by the invention is used for detecting the wheat drought resistance; the method is reliable, convenient and practical and has important application prospect in wheat genetic resource assessment and auxiliary selection of breeding markers; meanwhile, reference evidences are provided for cultivating of wheat varieties with strong drought resistance.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a molecular marker related to wheat drought resistance and its application, in particular to a primer related to wheat drought resistance, a molecular marker and its application. Background technique [0002] Wheat is an important grain crop and a strategic reserve grain variety in my country. With the vicious changes of the global climate and the intensification of human activities, the drought cycle is shortened and the degree is aggravated, which has an increasingly serious impact on wheat production. Breeding and promoting drought-resistant wheat varieties is one of the effective ways to ensure national food security and promote the sustainable and stable development of wheat production. The mining of drought resistance genes and the development of molecular markers are of great significance to the practice of wheat breeding. [0003] Adversity stresses such as drought will cause ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11A01H1/04
Inventor 张晓科穆培源张帆相吉山蒋雷王晓龙刘书慧
Owner NORTHWEST A & F UNIV
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