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Trichoderma preparation for controlling pepper phytophthora blight and field tank mix pesticide containing the same

A technology of Trichoderma and preparations, which is applied in the chemical, application, biocide and other directions for biological control, can solve the problems of uneven distribution of Trichoderma, difficult to preserve, and unsatisfactory control effect of pepper blight, and reduce the application of pesticides. The effect of frequency, long shelf life and good control effect

Inactive Publication Date: 2013-11-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this Trichoderma preparation, the Trichoderma on the wheat grains contains conidia and hyphae, and when applied in the field, the Trichoderma is unevenly distributed and difficult to preserve, and the control effect on pepper blight is not ideal at the same time

Method used

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  • Trichoderma preparation for controlling pepper phytophthora blight and field tank mix pesticide containing the same
  • Trichoderma preparation for controlling pepper phytophthora blight and field tank mix pesticide containing the same
  • Trichoderma preparation for controlling pepper phytophthora blight and field tank mix pesticide containing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Screening of Trichoderma Strains

[0048] The Trichoderma strain and Phytophthora capsici strain were cultured in a dish. In the experiment, the method of Melo et al. (2000) was used to inoculate the pre-activated Phytophthora capsici strain (5mm in diameter) into a PDA petri dish with a diameter of 90cm. The edge of the clump was 10mm away from the edge of the dish; after incubating at 25°C for 48h, inoculate A clump of pre-activated Trichoderma strain (5mm). There are three replicates for each Trichoderma strain, and a Phytophthora capsici medium plate not inoculated with Trichoderma strain is set as a control. After the confrontation culture was cultured in an incubator at 25°C for 4 days, observations were made.

[0049] Through the confrontation culture method, from the preliminary screening of 243 Trichoderma strains isolated in the laboratory, 4 strains with strong antagonism to Phytophthora capsici were selected, namely Trichoderma harzionum NF 9 , Tricho...

Embodiment 2

[0063] Example 2 Screening of fungicides

[0064] Thirteen different types of fungicides commonly used in production to prevent and control pepper blight were selected, and their inhibitory effects on the different developmental stages of Trichoderma mycelium, spore germination and spore production were determined. See Table 2 and Table 3 for the regression equation of virulence to mycelium and spore germination.

[0065] (1) The inhibitory effect of different fungicides on the growth of Trichoderma aculeatus Thz01 hypha

[0066] The mycelial growth rate assay method was used to determine the inhibitory effects of different fungicides on the growth of the hyphae of T. aculeatus Thz01, and the fungicides that did not inhibit the hyphae of T. aculeatus Thz01 were screened out.

[0067] Method: Firstly, determine the concentration range of the initial inhibition rate of each agent on the growth of Trichoderma hyphae, and then set 5 series of concentrations around this concentration. The...

Embodiment 3

[0092] Example 3 Preparation of Trichoderma preparation

[0093] The sodium naphthalenesulfonate formaldehyde condensate was purchased from Anyang Shuanghuan Auxiliary Co., Ltd., and lecithin was purchased from Zibo Panxin Pharmaceutical Chemical Co., Ltd.

[0094] (1) Inoculate Trichoderma aculeatus Thz01 on wheat kernel medium to produce spores;

[0095] (2) After the Trichoderma culture containing spores is naturally air-dried at 15°C to 25°C (or can be artificially dried) to a moisture content of 7-10%, then sieved to obtain Trichoderma spore powder.

[0096] (3) After mixing the sodium naphthalene sulfonate formaldehyde condensate, sodium lignin sulfonate, sodium lauryl sulfate, open powder, lecithin and diatomaceous earth, place them in a jet mill and grind them evenly. After 325-mesh sieve, add Trichoderma spore powder into a stirring tank in proportion to stir and mix well to obtain Trichoderma wettable powder (WP); wherein, the weight percentage of each component is:

[0097] ...

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Abstract

The invention discloses a trichoderma preparation for controlling pepper phytophthora blight and a field tank mix pesticide containing the same. The trichoderma preparation comprises, by weight: 28-32% of Trichoderma conidia, 10-15% of a dispersant, 4-6% of a wetting agent, 1-2% of a protective agent, and 50-55% of a carrier, wherein the trichoderma is Trichoderma. asperellum CGMCC No.6422. The field tank mix pesticide is prepared by mixing the trichoderma preparation and a bactericide and dissolving the mixture in water during application. The bactericide is eugenol or mandipropamid. The trichoderma preparation provided in the invention has the advantages of good dispersing effect, long storage period, and good pepper phytophthora blight control effect. In addition, the field tank mix pesticide developed in the invention can achieve a good control effect with a reduced number of times of pesticide delivery to phytophthora capsici, the control cost can be lowered, and pollution of reagents to the environment is reduced.

Description

Technical field [0001] The invention belongs to the technical field of biological control and chemical control of plant diseases, and particularly relates to a Trichoderma preparation for preventing and controlling pepper blight and a field barrel-mixed pesticide containing the Trichoderma preparation. Background technique [0002] Pepper blight is a soil-borne disease caused by Phytophthora capsici (Phytophthora capsici). The pathogen produces a large number of zoospores during the epidemic period, which can quickly lead to large-scale death of peppers and bring devastating losses to pepper production. [0003] How to effectively control pepper blight has always been a concern in production and research. In terms of disease-resistant breeding, no real breakthrough has been achieved in technology so far, and there are no real pepper blight-resistant varieties in production. At present, agricultural cultivation measures and chemical pesticide control are mainly used to control pepp...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P3/00A01N31/16A01N37/36
Inventor 张敬泽张艳丽张量
Owner ZHEJIANG UNIV
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