Polygonum multiflorum extract with excellent water solubility and preparation method thereof

Abstract

The invention discloses a polygonum multiflorum extract with excellent water solubility and a preparation method thereof. The preparation method comprises following steps: 1) polygonum multiflorum, which is taken as a raw material, is grinded and is subjected to reflux extraction with ethanol so as to obtain an extracting solution, the extracting solution is condensed and ethanol is recycled so as to obtain a concentrated solution, wherein the concentration of ethanol is 45-55%; 2) a compound enzyme is added into the concentrated solution, the mixture is subjected to enzymatic hydrolysis for 3 to 5h at a temperature of 40 to 50 DEG C, the compound enzyme is killed after enzymatic hydrolysis, the mixture is centrifuged, and the supernatant is collected, wherein the weight of the compound enzyme is 0.2 to 0.3% of the weight of the raw material; 3) the supernatant is filtered with a ceramic membrane of 0.4 to 0.5 microns, and the filtrate is collected; 4) the filtrate is eluted by using a macroporous resin column, wherein firstly the macroporous resin column is eluted with water until the effluent is clear, and then is eluted by ethanol with a concentration of 60 to 70%, the eluate is condensed and dried, and the polygonum multiflorum extract with excellent water solubility is obtained. The content of stilbene glucoside in the polygonum multiflorum extract is 5 to 60%. The preparation method is capable of reducing the content of anthraquinone compounds and increasing the extraction efficiency of stilbene glucoside.

Description

technical field [0001] The invention relates to the extraction of active components in plants, in particular to the extract of Radix Polygoni Multiflori with good water solubility and a preparation method thereof. Background technique [0002] Polygonum multiflorum, also known as Shouwu and Chishouwu, is the dry root tuber of Polygonum multiflorum thunb, a plant of the Polygonaceae family. Polygonum multiflorum mainly contains three types of active ingredients: stilbene glycosides, anthraquinones and polymerized proanthocyanidins. It also contains lecithin and various trace elements. Among them, the stilbene glycoside compounds are the unique components of Polygonum multiflorum, and the stilbene glycoside monomers that have been found so far include 2,3,5,4'-tetrahydroxystilbene-2-o-β-D-glucoside ( Referred to as stilbene glycoside), 2,3,5,4'-tetrahydroxy stilbene-2,3-di-o-β-D-glucoside (that is Polygonum multiflorum), 2,3,5,4 '-tetrahydroxystilbene-2-o-(6"-O-α-D-glucopyra...

AI Technical Summary

Problems solved by technology

And existing Polygonum multiflorum extract, if not through further separation, purification process (as crystallization, silica gel chromatography etc.), macromolecular substance and other impurity wherein are more, although stilbene glycoside compound itself has better Water-soluble, but due to the existence of other substances and a large number of impurities in the extract, the water-solubility of the extract is greatly reduced, so this kind of Polygonum multiflorum extract with low water solubility cannot be directly used in industrial production, and it is necessary to increase filtration or alcohol Processes such as dissolving, and increasing these processes may reduce the content of the original active ingredients in the extract while increasing the cost

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