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Preparation of dye-philic magnetic microsphere and application of dye-philic magnetic microsphere in separation of lactoferrin in whey

A technology of lactoferrin and magnetic microspheres, which is applied in the preparation methods of transferrin, peptides, animal/human proteins, etc., can solve the problems of high cost, complicated operation process and low product purity.

Inactive Publication Date: 2015-05-20
NORTHWEST A & F UNIV
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Problems solved by technology

Chromatography can obtain Lf with high purity, but its operation process is complicated and the cost is high; microfiltration / ultrafiltration is easy to operate and is suitable for mass production, but the product purity is low; salting-out precipitation method is relatively simple, but the product purity Not high, and will affect the activity of Lf

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  • Preparation of dye-philic magnetic microsphere and application of dye-philic magnetic microsphere in separation of lactoferrin in whey
  • Preparation of dye-philic magnetic microsphere and application of dye-philic magnetic microsphere in separation of lactoferrin in whey

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Embodiment Construction

[0029] It should be noted that the following embodiments are only preferred examples, and the present invention is not limited to these embodiments. For those skilled in the art, adding and replacing technical features within the scope of the present invention are all Belong to the protection scope of the present invention.

[0030] This example gives a preparation method of dye-affinity magnetic microspheres. Firstly, chitosan is modified on the surface of magnetic nanomaterials, and then reactive red 120 dye is connected to functionalize the surface, and the dye-affinity magnetic microspheres are obtained. ball, proceed as follows:

[0031] Step 1, preparation of magnetic nanomaterials (magnetic cores):

[0032] A) Preparation of Fe by chemical co-precipitation method 3 o 4 Nanoparticles. The mass ratio formula of each reagent is: FeCl 3 ·6H 2 O: FeCl 2 4H 2 O: H 2 O=2.3751:0.8735:250;

[0033] B) Weigh FeCl 3 ·6H 2 O and FeCl 2 4H 2 O, dissolved in deionized w...

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Abstract

The invention discloses a preparation method of a dye-philic magnetic microsphere and application of the dye-philic magnetic microsphere in separation of lactoferrin in whey. According to the invention, dye-philic adsorbent having a separation function is prepared on the surface of a magnetic Fe3O4 nano particle, and the method comprises the following steps: preparing a magnetic chitosan microsphere, and functionalizing a reactive red 120 dye. The affine adsorbent obtained after functionalizing the surface of the Fe3O4 nano particle has a function of separating lactoferrin in whey, and the dye-philic magnetic microsphere can be used repeatedly.

Description

technical field [0001] The invention belongs to the technical field of food separation, and relates to the separation of lactoferrin in whey, in particular to the preparation of a dye-affinity magnetic microsphere and its application for the separation of lactoferrin in whey. Background technique [0002] Lactoferrin (Lactoferrin, Lf) is a natural active glycoprotein with broad-spectrum antibacterial, antiviral, immune regulation and other biological functions. Due to the immature immune system of infants and young children, their anti-infection ability is weaker than that of children and adults, and they are prone to intestinal problems that affect nutrient intake, which in turn affects growth and development. Adding Lf to infant formula can help infants resist microorganisms such as E. coli and reduce the incidence of diarrhea. As an important ingredient of infant milk powder, Lf has a huge market demand, but high-purity Lf is expensive. The main reason is that the separa...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/24B01J20/28B01J20/30C07K14/79C07K1/14
Inventor 李忠宏曹淼岳田利葛武鹏王建龙李荣华段旭昌
Owner NORTHWEST A & F UNIV
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