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A controllable and versatile method for directional surface imprinting and applications of the resulting molecularly imprinted polymers

A molecular imprinting and surface imprinting technology, applied in chemical instruments and methods, ion exchange, ion exchange regeneration, etc., can solve problems such as poor controllability and versatility, and achieve large binding capacity, fast binding and analysis speed, and imprinting efficiency. high effect

Active Publication Date: 2016-08-10
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the shortcomings of poor controllability and versatility of the existing western imprinting technology, the purpose of the present invention is to provide a controllable and universal directional surface imprinting method and the application of the resulting molecularly imprinted polymer

Method used

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  • A controllable and versatile method for directional surface imprinting and applications of the resulting molecularly imprinted polymers
  • A controllable and versatile method for directional surface imprinting and applications of the resulting molecularly imprinted polymers
  • A controllable and versatile method for directional surface imprinting and applications of the resulting molecularly imprinted polymers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Preparation of polymer-modified glass flakes

[0031] First prepare the pre-polymerization solution: the pre-polymerization solution is a 0.1M, pH8.5 phosphate buffer containing 2.0mg / mL dopamine, 1.6mg / mL m-aminophenylboronic acid and 1.2mg / mL ammonium persulfate. After the pre-polymerization solution is mixed evenly, glass flakes are added, and polymer-modified glass flakes can be obtained by self-polymerizing at room temperature for 24 hours. The result is as figure 2 b. with untreated glass ( figure 2 A) Contrast, showing a noticeably darker color.

Embodiment 2

[0032] Example 2: Contact Angle Test of Polymer Modified Glass Sheets

[0033] The cleaned untreated glass piece and the glass piece obtained in Example 1 were dried and used for the contact angle test respectively. The results are as follows figure 2 C, 2D. Among them, the contact angle of the polymer-modified glass sheet was 38.9°, and that of the untreated glass sheet was 66.1°. Obviously, the polymer-modified glass flakes are more hydrophilic.

Embodiment 3

[0034] Embodiment 3: the investigation of the relationship of polymer thickness with time

[0035] Place a group of clean glass slides in 0.1M, pH 8.5 phosphate buffer containing 2.0mg / mL dopamine, 1.6mg / mL m-aminophenylboronic acid and 1.2mg / mL ammonium persulfate, and control the polymerization time respectively for 0.5h, 1.0h, 1.5h, 2.0h and 2.5h. The aggregate thickness was then characterized by atomic force microscopy (AFM). The result is as image 3 . In the figure, the linear correlation coefficient r2=0.95, and the slope s=3.5±0.4nm / h, which shows that the thickness of the polymer has a good linear correlation with the time growth, which provides a guarantee for the controllability of this technology.

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Abstract

The invention discloses a controllable and universal directional surface printing method. The method comprises the steps as follows: template glucoprotein is anchored on the surface of a substitution boric acid functional substrate material via boron affinity interaction, the polymer formed by auto-polymerization in water phase of a compound capable of automatically polymerizing forms a printing layer, and a template is eluted under the acid condition to form a printing hollow cavity, as a result, the molecular printing polymer is obtained. The molecular printing polymer prepared via the method not only has the advantages of good specificity, high combination capacity, strong anti-interference capability, quick combination and analysis speed and the like, but also can exclusively identify the template glucoprotein with or without the boron affinity interaction, is adjustable in force of combination with the template when keeping the specificity, and is wide in application range. The method has the advantages of convenience, controllability, universality, high printing efficiency and the like. The molecular printing polymer can exclusively identify, gather, separate and detect the glucoprotein with low enrichment in a complex practical sample.

Description

technical field [0001] The invention relates to the field of molecularly imprinted functionalized materials, specifically a controllable and universal directional surface imprinting method and the application of the obtained molecularly imprinted polymer in the specific recognition, enrichment, separation and detection of glycoproteins. Background technique [0002] Glycoproteins are a class of proteins with important biological functions and clinical significance. They are ubiquitous in living organisms, for example, more than half of the total proteins in mammals are glycoproteins. Glycoproteins play important roles in physiological processes such as molecular recognition, immune response, intra / intercellular signaling, and developmental regulation. In addition, glycoprotein is also of great significance in the early diagnosis of major diseases, such as infection, tumor, cardiovascular disease, liver disease, kidney disease and diabetes, etc. Most of the disease markers ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26B01J20/30B01D15/08
Inventor 刘震王双寿毕晓东
Owner NANJING UNIV
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