pH indicator containing water-soluble indocyanine derivatives and its application
An indicator, the technology of indocyanine, which is applied in the field of pH indicator of indocyanine derivatives, can solve the problems that pH indicators are rarely reported, and achieve the effects of anti-interference from environmental factors, less invasiveness, and broad application prospects
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Embodiment 1
[0047] Example 1: Extremely basic pH indicator of carboxylate functionalized indole squaraine dye.
[0048] 1) Indole squaraine dye 1 (1×10 -6 M), whose structural formula is as follows, were added to Tris-HCl buffer solutions with pH values from 8 to 13, mixed thoroughly and left to stand for 10 minutes. Test its ultraviolet absorption spectrum and fluorescence emission spectrum respectively, such as figure 2 shown. The apparent color of indole squaraine dye 1 is blue between pH 8-11, and red under ultraviolet light; when the pH value is greater than 11, the apparent color gradually changes from blue to colorless, and it is The light gradually changes from red to yellow. The absorption peak at the maximum absorption peak of the ultraviolet absorption spectrum at 637nm decreases rapidly when the pH value is greater than 11, and a new absorption peak appears at 396nm and gradually increases; the corresponding fluorescence emission intensity decreases gradually at 647nm, ...
Embodiment 2
[0053] Example 2: Extremely basic pH indicator of primary amino functionalized indole squaraine dye.
[0054] 1) The implementation steps 1-4 are the same as the implementation example 1, and the structural formula of indole squaraine 2 is shown below.
[0055] UV absorption and fluorescence emission spectra of dye 2 with a pH interval of 1 such as Image 6 shown. It is found that dye 2 and dye 1 have the same spectrum change law through the law of ultraviolet and fluorescence intensity. Between 10.6 and 13 with a pH interval of 0.2, the jump range for dye 2 is smaller than the pH required for dye 1, as Figure 7 shown. By calculating its pKa value, the pKa value obtained from the ultraviolet absorption spectrum is 11.29±0.01, and the pKa value obtained from the fluorescence emission spectrum is 11.28±0.04. Combined with the above spectral characteristics of dye 2, its color change range is smaller than that required by dye 1.
[0056]
Embodiment 3
[0057] Example 3: Extremely basic pH indicators of carboxylate functionalized indocyanine dyes.
[0058] 1) The implementation of steps 1-4 is the same as that of Example 1, and the structural formula of the indocyanine dye 3 is shown below. The change rule of its spectral properties is basically the same as that of dye 1, but its pKa value is larger than that of dye 1, so its color changing range is larger than the pH value required by dye 1. The pKa value calculated from the UV absorption was 12.68±0.01, and the pKa value calculated from the fluorescence emission was 12.67±0.04.
[0059]
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