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Phase-shifting image digital holographic microscope system and microscopy method thereof

A technology of digital holographic microscopy and microscopy systems, applied in microscopes, measuring devices, instruments, etc., can solve problems such as inability to phase compensation, and achieve the effect of reducing operational accuracy requirements

Inactive Publication Date: 2013-11-27
SUZHOU UNIV
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Problems solved by technology

[0006] The phase-shift holographic technology in the form of image plane cannot effectively compensate the phase

Method used

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  • Phase-shifting image digital holographic microscope system and microscopy method thereof
  • Phase-shifting image digital holographic microscope system and microscopy method thereof

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Embodiment Construction

[0041] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.

[0042] A digital holographic microscope system with image plane phase shift of the present invention comprises:

[0043] a laser for generating a laser beam;

[0044] The beam splitting unit is used for splitting the laser beam generated by the laser into an illumination beam and a reference beam. In the present invention, the beam generated by the laser is split by the beam splitting unit, and the illumination beam and the reference beam ...

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Abstract

The invention discloses a phase-shifting image digital holographic microscope system and a microscopy method thereof. The microscope system comprises a laser device, a beam dividing unit, a lighting beam transmission unit, a reference beam transmission unit, a beam combining nit and an imaging unit. The reference beam transmission unit comprises a phase-shifting device used for leading in phase shifts in reference beams. According to the phase-shifting image digital holographic microscope system and the microscopy method thereof, a phase-shifting image microscopy holographic technique is adopted, a holographic picture of an image plane is directly recorded, and phase compensation can be carried out through software to obtain precise phase information of an object.

Description

technical field [0001] The invention relates to the technical field of digital holographic microscopy, in particular to an image plane phase-shifting digital holographic microscopy system and a microscopic method thereof. Background technique [0002] At present, ordinary microscopes can only measure the intensity information of objects but not the phase information, and usually when observing biological cells, dyeing operations are required, which may destroy the structure of biological cells. [0003] The lyncee company in Switzerland invented and produced an off-axis digital holographic microscope, which records the hologram of an out-of-focus object and uses a computer to reproduce it to measure the phase information of the object. Digital holographic microscopes in the prior art have the following disadvantages: [0004] When recording off-axis, the resolution is limited and accurate measurement of the reconstruction distance is required; [0005] The existing phase-s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01J9/02G02B21/00G02B21/06G02B21/18G02B21/36G03H1/10
Inventor 周皓顾济华赵宏波陈大庆
Owner SUZHOU UNIV
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