Pig HOXA10 gene segment as genetic marker of pig litter size traits and application
A genetic marker, litter size technology, used in DNA/RNA fragmentation, recombinant DNA technology, determination/inspection of microorganisms, etc.
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Embodiment 1
[0023] Example 1: The acquisition of gene fragments and the establishment of polymorphism detection methods
[0024] Sequence amplification of the first exon of porcine HOXA10 gene
[0025] (1) Collect ear tissue samples
[0026] A foreign pig breed (Large White pig) and a Chinese pig breed (Meishan pig, from the experimental pig farm of Huazhong Agricultural University, Wuhan City, Hubei Province, China) were selected as test materials, and the ear margin tissues of piglets were collected on the day of piglet birth;
[0027] (2) Genomic DNA extraction
[0028] Genomic DNA of pigs was extracted from pig ear margin tissue (method referred to the method introduced by Xiong Yuan, "Introduction to Pig Biochemistry and Molecular Genetic Experiments", China Agricultural Press, 1999).
[0029] (3) Primer design
[0030] A pair of specific primers were designed according to the first exon sequence of porcine HOXA10 gene (GenBank gene accession number: JN_836600). in
[0031] The ...
Embodiment 2
[0037] Example 2: Detection of polymorphism distribution of genetic markers prepared by the present invention in different pig herds
[0038] The PCR-RFLP method was used to detect the genotype and allele frequency distribution of the PCR-MslI-RFLP polymorphism site in 844 pigs of 6 breeds at home and abroad. The results are shown in Table 1. It includes local Chinese pig breeds Meishan pig, Tongcheng pig and Tibetan pig, and foreign pig breeds include Landrace pig, Large White pig and Duroc pig. The test results showed that the three genotypes of this polymorphic site, namely TT, CC and TC genotypes, were distributed in Landrace pigs, Large White pigs and Tongcheng pigs, but not detected in Meishan pigs, Duroc pigs and Tibetan pigs to the CC genotype. Among them, the C allele is clearly dominant in Large White pigs; the T allele frequencies in Meishan pigs, Duroc pigs and Tibetan pigs are about 90%, 79% and 60% respectively; The allele frequency was slightly higher than tha...
Embodiment 3
[0044] Example 3: Association analysis of genetic markers prepared by the present invention and litter size traits
[0045] In order to determine whether the pig HOXA10 gene PCR-MslI-RFLP is related to the pig phenotype difference, the applicant used SAS software to analyze the polymorphism site of the pig HOXA10 gene exon PCR-MslI-RFLP and the Guangdong Wenshi Shuitai breeding pig group Preliminary correlation analysis was carried out on reproductive traits such as total litter size and live litter size of 302 Landrace pigs. The models used are:
[0046] yi=μ+αi+bj+pk+ei
[0047] yi represents the trait value, μ represents the overall mean, αi represents the genotype effect value (i=TT, TC, CC),
[0048] bj represents the breed effect (j=1,2), pk represents the fixed effect of the kth parity, and ei represents the random error.
[0049] There were 114 CC genotypes, 109 TT genotypes, and 79 TC genotypes of this polymorphic site in 302 Landrace pigs. The results of statisti...
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