Surface-enhanced Raman substrate and preparation method thereof

Abstract

This invention relates to the technical field of Raman spectrum molecule detection, and discloses a surface-enhanced Raman substrate and a preparation method thereof. According to the present invention, a signal strength is enhanced through holes aligned on the surface of a substrate and having a periodical lattice structure so as to increase sensitivity and reduce detection limitation; and the technology that holes are punched on a substrate is a mature technology in a semiconductor manufacturing process, and the surface-enhanced Raman substrate has characteristics of low cost, simple process, easy operation, good Raman signal enhancement and good repeatability.

Description

Technical field [0001] The invention relates to the technical field of Raman spectroscopy molecular detection, in particular to a surface enhanced Raman substrate and a preparation method thereof. Background technique [0002] The development of life sciences has entered the era of detection and manipulation at the single cell and single molecule level. Because the optical method can directly image, and has the least interference to the research object, it has the advantages of non-destructive, in-situ measurement, etc., and has become the preferred research method for life sciences. Raman spectroscopy can obtain molecular vibrational energy level information, which is called molecular "fingerprint spectroscopy", which can provide detailed structural information of molecules, such as the type, strength, angle, and conformational changes of chemical bonds. However, the efficiency of ordinary Raman scattering is very low. Under normal circumstances, the Raman scattering cross sect...

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Problems solved by technology

This method is simple, but it is mostly only suitable for specific excitation wavelengths, and the sol is a metastable system, which is easy to aggregate for a long time, so the prepared substrate has poor reproducibility

To use the self-assembly method to make SERS active substrates with controllable surface plasmon resonance, there are usually two methods. One is to change the shape of the nano-sol, such as using a composite structure with adjustable parameters. By changing the shape of the nanoparticles, surface plasmons can be obtained. Nanoparticles with controllable resonance, and then assemble them into films to obtain nanofilms with different plasmon resonances. In this respect, the core-shell structure prepared by researchers at Rice University in the United States is more representative, but this method is relatively cumbersome. , and has the disadvantage of being difficult to control by chemical methods; another method is to change the degree of aggregation of nano-sols on the substrate, that is, to change the density of nanoparticles on the substrate. Repeatedly immersing the gold film several times to reduce the repulsion between gold nanoparticles to obtain SERS active substrates with different gold sol densities. This method has a limited controllable range of plasmon resonance and is time-consuming, usually 3-4 days

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