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Composition and method for promoting hair growth

A technology of hair growth and composition, which is applied in the field of hair growth-promoting composition, and can solve the problem that the hair growth-promoting effect is not fully satisfactory

Inactive Publication Date: 2013-12-18
HSIANG MAO LIFE SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its hair growth effect is still not fully satisfactory

Method used

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  • Composition and method for promoting hair growth
  • Composition and method for promoting hair growth
  • Composition and method for promoting hair growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1─In vitro culture of hair follicle pilomastoid cells

[0034] Eight-week-old male SD rats were purchased from the Experimental Animal Center of the National Science Council. Hair follicles and pilomastoids were obtained from rat vibrissae follicles by microsurgery under a dissecting microscope. The zeroth generation hair follicle pilomastoid cells were cultured in 35mm culture dishes (purchased from Falcon, Bedford, MA, USA), supplemented with RPMI-1640 medium (purchased from Invitrogen, Carlsbad, CA, USA), and supplemented with 10% Fetal bovine serum (purchased from Hyclone, Logan, Utah, USA), 2 mM L-glutamic acid, 50 IU / ml penicillin and 50 μg / ml streptomycin. Hereinafter, the RPMI-1640 medium supplemented with serum is referred to as medium A, while the RPMI-1640 medium without serum supplemented is referred to as medium B for short. When the cells grew to 80% full, they were re-cultivated in 100mm culture dishes (purchased from Falcon, Bedford, MA, USA...

Embodiment 2

[0035] Example 2 - Synergistic effect of EGCG and adenosine

[0036] In a 24-well culture dish (purchased from Falcon, Bedford, MA, USA), 0.5 ml of dermal papilla cells obtained in Example 2 were planted in each well, and the cell density was 1×10 4 / ml. Add gallocatechin epigallate (EGCG) (purchased from Sigma-Aldrich, St.Louis, MO, USA) and / or adenosine (purchased from Sigma-Aldrich, St.Louis, MO, USA) media, to reach final concentrations of 0.5 μM and 5 μM, respectively. at 37°C and 5% CO 2 After culturing for 24 hours under the condition of , stain with 0.4% trypan blue solution to count the number of dermal papillae cells. The same experiment was repeated 4 times and the results are shown in Table 1 below.

[0037] Table 1

[0038]

[0039] The unit of cell number in Table 1 is 1×10 4 / ml

[0040] As shown in Table 1, if the number of dermal papilla cells cultured only in medium A is set as 100%, EGCG alone can increase the number of cells by about 17%, but ade...

Embodiment 3

[0041] Example 3 - Preparation of supernatant of hair follicle pilomastoid cell culture

[0042] 10ml of hair follicle dermal papilla cells (1x10 6 cells / ml) into 100-mm culture dishes. After 24 hours of incubation in Medium A, the culture supernatant was collected and centrifuged at 3000 x g for 15 minutes at 4°C to remove cell debris. The resulting liquid was filtered through a filter membrane with a pore size of 0.2 μm to obtain supernatant C, which was stored at -70°C for future use.

[0043] On the other hand, 10ml of hair follicle pilomastoid cells (1x10 6 cells / ml) were seeded into 100-mm culture dishes and cultured in medium A until the next day. Remove the medium A, and then wash the cells with RPMI-1640 medium 3 times, 5 minutes each time. Serum-free culture supernatants were produced by culturing with 10 ml of RPMI-1640 medium for at least 8 hours. Culture supernatants were collected and centrifuged at 3000 x g for 15 minutes at 4°C to remove cell debris. The ...

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Abstract

The invention discloses a composition and a method for promoting hair growth. The composition comprises flavonoid derivatives and an adenosine compound and especially comprises catechin derivatives and adenosine as active ingredients, and a cosmetic or a pharmaceutically acceptable carrier. In a better embodiment of the invention, the composition comprises a supernatant from a hair follicle mastoid cell culture.

Description

【Technical field】 [0001] The present invention relates to a composition and method for promoting hair growth. The composition of the present invention contains flavonoid derivatives and adenosine compounds, especially catechin derivatives and adenosine, as active ingredients, and a cosmetically or pharmaceutically acceptable carrier. In a preferred embodiment of the present invention, the composition of the present invention further comprises a supernatant from dermal papilla cell culture of hair follicles. 【Background technique】 [0002] The clinical manifestation of hair loss is baldness or alopecia. Although such symptoms may not cause substantial physical discomfort, they may cause psychological hindrance due to personal aesthetic requirements. Clinically, taking drugs, skin trauma, skin lesions, nutritional disorders, metabolic disorders, endocrine abnormalities, high fever, etc. may cause hair loss. [0003] Hair is generally produced by hair follicles. Human hair ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/60A61K8/49A61K8/97A61K8/98A61K31/7076A61K36/82A61P17/14A61Q7/00A61K31/353A61K35/36
Inventor 夏静吾
Owner HSIANG MAO LIFE SCI & TECH CO LTD
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