Preparation method and application of mammal bone marrow intracellular fluid

Abstract

The invention discloses a preparation method and application of mammal bone marrow intracellular fluid. The preparation method comprises the following steps: adding fresh isolated bone marrow cells into normal saline, wherein concentration is 108 to 109cell / ml; then repeatedly freezing and thawing to crack the bone marrow cells; and separating cell membranes from intracellular fluid by high speed centrifugation, then extracting surface supernatant, filtering to the aseptic state so as to obtain filtrate, i.e. the mammal bone marrow intracellular fluid, wherein the fresh isolated bone marrow cells are from a mammal. Current, tumor treatment modes comprise drug treatment, operative treatment and radiotherapy; the drug treatment further comprises chemical drug treatment, biological treatment and gene therapy, but the chemical drug treatment, the biological treatment and the gene therapy all cannot achieve a satisfied treatment effect; and particularly, chemotherapy is used as the main treatment method, but has a particularly serious killing effect on a human body, and thus, usually, tumor is not inhibited, but a patient is died. The mammal bone marrow intracellular fluid prepared by the method disclosed by the invention has no toxic or side effects on the body and has an obvious tumor inhibiting effect.

Description

technical field [0001] The invention relates to the technical field of clinical medicine, in particular to a preparation method and application of mammalian bone marrow intracellular fluid. Background technique [0002] When a gene mutation occurs in a cell, the cell will proliferate abnormally and will not be controlled by the body's immune system, eventually leading to malignant transformation of the cell and forming a malignant tumor. Malignant tumors can invade and destroy adjacent tissues and organs, and cancer cells can multiply and spread rapidly. It is one of the most important diseases that threaten human life and health at present. With the improvement of technology and living standards, people pay more and more attention to the quality of life, and there is an urgent need for a targeted, safe, and less toxic and side effect treatment method in the clinical treatment of tumors. The treatment of tumors includes drug therapy, surgical therapy and radiation therapy. ...

AI Technical Summary

Problems solved by technology

At present, many researchers turn to the research on cell biological therapy. Some studies have reported that the disorder of protein tyrosine kinase function will cause a series of diseases in the organism. More than 50% of the proto-oncogene and oncogene products have protein tyrosine kinase. activity, their abnormal expression will lead to the disorder of cell proliferation regulation, and then lead to tumorigenesis

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