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Method for detecting gonococcocci in female genital secretion and reaction pad used by method

A technology for reproductive tract and gonorrhoeae, which is applied in the direction of analyzing materials through chemical reactions and observing the impact on chemical indicators. It can solve the problem of insufficient stability of oxidase detection, inconvenient storage and work, and inapplicability. Clinical screening and other issues, to achieve the effect of convenient clinical rapid screening, sensitive detection method, and not easy to be oxidized

Active Publication Date: 2013-12-25
SHANDONG MAITIAN BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, since the tetramethyl-p-phenylenediamine hydrochloride reagent can quickly oxidize itself and lose its sensitivity, the current method is not stable enough for oxidase detection, resulting in low sensitivity and inconvenience to storage and work, and is not suitable for clinical screening. check

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Drop 1 μl of oxidase reagent A on the blank reaction pad, dry it with flowing air for 4 hours, add 1 μl of auxiliary reagent C to the reaction pad, and dry it with flowing air for 2 hours to obtain reaction pads A-C.

Embodiment 2

[0038] Drop 1 μl of oxidase reagent B on the blank reaction pad, dry it with flowing air for 4 hours, add 1 μl of auxiliary reagent C to the reaction pad, and dry it with flowing air for 2 hours to obtain reaction pads B-C.

Embodiment 3

[0040] Drop 1 μl of oxidase reagent C on the blank reaction pad, dry it with flowing air for 4 hours, add 1 μl of auxiliary reagent C to the reaction pad, and dry it with flowing air for 2 hours to obtain reaction pads C-C.

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Abstract

The invention relates to a method for detecting gonococcocci in female genital secretion and a reaction pad used by the method, which belong to the technical field of biological chemistry detection. The reaction pad is provided with liquor (an oxidase reagent) prepared by mixing 0.1-2mg\ml ascorbic acid and 10-12mg\ml hydrochloric tetramethyl-p-phenylenediamine in a volume ratio of 1:500, and a 0.1-1mg / ml blue vitriol solution (an auxiliary reagent), wherein the volume ratio of the oxidase reagent to the auxiliary reagent is 1:1. The ascorbic acid with certain concentration is added into an oxidase substrate, so that the stability of the hydrochloric tetramethyl-p-phenylenediamine is enhanced, and further, the reaction pad is unlikely to be oxidized and convenient to store; the auxiliary reagent, namely the blue vitriol, acts between the substrate and a sample as a color reagent, so that the detection method is flexible, and fast clinic screening is convenient.

Description

technical field [0001] The invention relates to a method for detecting Neisseria gonorrhoeae in female reproductive tract secretions and a reaction pad used, belonging to the technical field of biochemical detection. Background technique [0002] Reproductive tract infection is a disease that seriously threatens women's physical and mental health. When women's immune function is weakened, endocrine function is disordered, the natural defense system of the vagina is destroyed, the beneficial bacteria in the vaginal microecology decrease, and the invasion of pathogenic bacteria leads to the occurrence of infectious diseases. Worldwide, especially in developing countries, the proportion of women suffering from reproductive tract diseases is increasing. Common reproductive tract infection diseases include bacterial vaginosis (invasion of anaerobic bacteria such as Gardnerella vaginalis and Bacteroides), Vaginitis (invasion of Candida albicans), trichomonal vaginitis (invasio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 宋筱亮丁芬
Owner SHANDONG MAITIAN BIOLOGICAL TECH CO LTD
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