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Method for constructing rapid hepatotoxicity predication model of phenolic compound

A phenolic compound and construction method technology, which is applied in the field of construction of a rapid prediction model of phenolic compound hepatotoxicity, can solve problems such as affecting normal function, abnormal expression of cytochrome P450, damage, etc., and achieves the effect of reliable indicators

Inactive Publication Date: 2015-07-15
SHANGHAI JIAOTONG UNIV
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  • Description
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  • Application Information

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Problems solved by technology

[0002] In daily life, many phenolic compounds ingested by people have the hazard of causing liver damage. However, there are many response indicators after liver damage. For example, liver damage will lead to the depolarization of the mitochondrial potential of cells, affecting normal functions, and causing excessive production of ROS free radicals. and DNA damage, abnormal expression of cytochrome P450, etc., but these indicators are not comprehensive
Therefore, it is inaccurate to simply use one indicator to judge

Method used

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  • Method for constructing rapid hepatotoxicity predication model of phenolic compound
  • Method for constructing rapid hepatotoxicity predication model of phenolic compound
  • Method for constructing rapid hepatotoxicity predication model of phenolic compound

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Experimental program
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Embodiment 1

[0029] 1. Select 6 common phenolic compounds such as gallic acid, nordihydroguaiaretic acid (NDCG), green tea, quercetin, caffeic acid, epigallocatechin gallate (EGCG), and the concentration of monomers It is 10mg / ml, and the extract concentration is 100mg / ml. Prepare the mother solution and dissolve it with dimethyl sulfoxide (DMSO) to ensure that the DMSO concentration is controlled below 0.5%.

[0030] 2. The cell plating density is 20000 / well, 200 μl, after 24 hours, suck out the supernatant, add 200 μl of drug, add rhodamine 123 with a final weight volume concentration of 5 μg / ml after 2.5 hours of action, and remove the supernatant after 30 minutes Aspirate, wash with HBSS, then add 200 μl of drug, and detect with an excitation wavelength of 490 nm and an absorption wavelength of 535 nm on a microplate reader after 24 hours. Test results such as figure 1 shown.

[0031] 3. ROS generation rate experiment: the cell plating concentration was 20000 / well, 200 μl, after 24 ...

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Abstract

The invention belongs to the technical field of rapid toxic substance detection, and particularly relates to a method for constructing a rapid hepatotoxicity predication model of a phenolic compound. According to the method, an HepG2 / C3A cell with stable inheritance and high-expression cytochrome P450 is taken as a research object; 6 types of natural common phenolic compounds are selected and act on the cell respectively, and indexes such as cell CYPIA mitochondrial depolarization, ROS production rate and the like are tested respectively; and statistical analysis is combined, and finally, the prediction model is established. The method is simple and rapid and is used for predicating the hepatotoxicity of a drug, the result is stable, the accuracy is improved, and the method has a potential popularization and application prospect.

Description

technical field [0001] The invention belongs to the technical field of rapid detection of toxic substances, and in particular relates to a method for constructing a rapid prediction model of hepatotoxicity of phenolic compounds. Background technique [0002] In daily life, many phenolic compounds ingested by people have the hazard of causing liver damage. However, there are many response indicators after liver damage. For example, liver damage will lead to the depolarization of the mitochondrial potential of cells, affecting normal functions, and causing excessive production of ROS free radicals. And DNA damage, abnormal expression of cytochrome P450, etc., but these indicators are not comprehensive. Therefore, it is inaccurate to judge simply by one indicator. [0003] The present invention constructs a simple and fast prediction method by screening the response indicators of liver damage caused by phenolic compounds in the early stage, combining the stable genetic charact...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/63
Inventor 陆维盈刘洁史海明俞良莉
Owner SHANGHAI JIAOTONG UNIV