Method used for modifying collagen with epoxy quaternary ammonium salt

Abstract

The invention discloses a method used for modifying collagen with an epoxy quaternary ammonium salt. The method is characterized in that: quaternary ammonium salt type collagen is prepared by reacting the epoxy quaternary ammonium salt with collagen, so that hydrophilcity of collagen is improved, and collagen will possess a certain degree of antibacterial properties. The method comprises following steps: collagen and the epoxy quaternary ammonium salt are reacted for 1 to 24h at a temperature of 0 to 45 DEG C according to a certain ratio, and then the mixture is washed with water; 0.1 to 2 weight portions of an amino acid is added for 0.5 to 1h of reaction at a temperature of 0 to 45 DEG C, and then the product is washed with water; 0.5 to 3.5 weight portions of an acid or an acid salt is added so as to adjust the pH value to 6.5 to 7.5; and finally 100 to 500 weight portions of water is added, and the final product is washed with water at a temperature of 0 to 45 DEG C for 10 to 30min, and the procedure is repeated for 5 times.

Description

technical field [0001] The invention relates to a method for modifying collagen with epoxy quaternary ammonium salt. It belongs to the field of medical biomaterials. Background technique [0002] Collagen is the main structural protein of connective tissue, accounting for about 1 / 3 of the total protein in mammals, and widely exists in animal skin, bone, cartilage, tendon, ligament and blood vessel wall, etc., in the form of three-dimensional network of collagen fibers exist. Collagen has many advantages such as low immunogenicity, low irritation, low cytotoxicity, good biocompatibility, good performance of promoting cell growth and biodegradability, etc., and it is widely used in the field of biomedicine. [0003] Since collagen itself does not have antibacterial / bacteriostatic properties. When collagen is used as a biological dressing, because the wound is a warm and humid environment, bacteria multiply on the wound, especially for some wounds that are not completely deb...

AI Technical Summary

Problems solved by technology

[0008] The present invention is designed for the deficiencies of collagen in antibacterial/antibacterial aspects, and it overcomes the shortc