Method for identifying plant-derived waste oil using trace fatty acids
A plant-derived, fatty acid technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as missed judgments, and achieve the effect of improving accuracy and specificity
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Embodiment 1
[0023] Take 0.06g of soybean oil, corn oil, peanut oil and other natural vegetable oils and the heated samples of the first three natural vegetable oils and place them in disposable polytetrafluoroethylene tubes, and then add 150 μL of 2mol / L potassium hydroxide methanol solution to them respectively, and then respectively Add 1mL of n-hexane, shake well, and let stand to separate layers. Take 1 μL of the supernatant, respectively, for gas chromatography and multidimensional gas chromatography for analysis. After the analysis by the instrument, the normalization method was used to process the data, and the relative content (M0) of the saturated linear pentadecyl fatty acid methyl ester was recorded on the gas chromatograph. Then combined with the peak area ratio (A1 / A0) of the isomerized 15-carbon fatty acid methyl ester and the saturated straight-chain 15-carbon fatty acid methyl ester in the multidimensional gas chromatograph, the relative ratio of the isomerized 15-carbon f...
Embodiment 2
[0028] Take 0.06g of oil samples from different sources and place them in disposable polytetrafluoroethylene tubes, then add 150μL of 2mol / L potassium hydroxide methanol solution to it, and then add 1mL of n-hexane respectively, after fully shaking, let stand to separate Floor. Take 1 μL of the supernatant, respectively, for gas chromatography and multidimensional gas chromatography for analysis. After the analysis by the instrument, the normalization method was used to process the data, and the relative content (M0) of the saturated linear pentadecyl fatty acid methyl ester was recorded on the gas chromatograph. Then combined with the peak area ratio (A1 / A0) of the isomerized 15-carbon fatty acid methyl ester and the saturated straight-chain 15-carbon fatty acid methyl ester in the multidimensional gas chromatograph, the relative ratio of the isomerized 15-carbon fatty acid methyl ester in different animal oils was calculated. Content (M1), M1=A1 / A0×M0.
[0029] According t...
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