Constant-cold protection system for tissue homogenate extraction

Abstract

The invention relates to a constant-cold protection system for tissue homogenate extraction. The constant-cold protection system for the tissue homogenate extraction is characterized by comprising a cooler and a cooling source, wherein a centrifugal tube base is arranged in an inner chamber of the cooler; cooling liquid is filled into the cooler; the cooler is communicated with the inner chamber of the cooling source through a first cooling water outlet pipe and a second cooling water inlet pipe respectively; the cooling source comprises a cooling source container, a semiconductor refrigerator, a radiator and a filter; a cold terminal of the semiconductor refrigerator is arranged at the bottom of the cooling source container; a hot terminal of the semiconductor refrigerator is connected with the radiator in a heat-conducting way; a cooling liquid flow speed control pump is arranged on a pipeline of the first cooling water outlet pipe; the cooler is provided with a first temperature sensing circuit; a second temperature sensing circuit is inside the cooling source container; and the first temperature sensing circuit, the second temperature sensing circuit, the cooling liquid flow speed control pump and the semiconductor refrigerator are electrically connected with a controller respectively. The constant-cold protection system for the tissue homogenate extraction has the characteristics of good homoiothermy, capabilities of ensuring that the tissue extracting process is always in the low-temperature state, avoiding changes of properties of tissue protein and nucleic acid and improving the work efficiency, and the like.

Description

technical field [0001] The invention relates to a constant temperature device, in particular to a constant cold protection system for tissue homogenate extraction. Background technique [0002] Tissue homogenization is a laboratory technique frequently used in modern life science research. In the process of protein extraction and nucleic acid extraction, the tissue or cells are ground by a tissue homogenizer to destroy the original structure of the tissue and cells, and extract the target tissue protein or nucleic acid. These operations often need to be performed at low temperatures to prevent damage such as tissue protein denaturation or nucleic acid condensation. The homogenizer commonly used in current laboratories grinds the homogenized tissue through mechanical rotation. During this process, the grinding head of the homogenizer will inevitably generate heat through mechanical friction, and at the same time, the friction between the grinding head and the tissue will al...

AI Technical Summary

Problems solved by technology

In order to prevent the temperature rise from destroying the protein and nucleic acid structure of the tissue, in the process of extracting tissue with the current experimental homogenizer, it is often necessary to repeatedly intermittently operate, and the tip of the homogenizer is taken out of the tissue homogenate, so that the homogenizer and the tissue cool down, avoiding temperature Elevation leads to protein denaturation and affects the activity of extracted tissues

Due to the manual operation, it cannot guarantee that the tissue extraction process is always in a low temperature state, which will change the properties of tissue protein and nucleic acid, and affect work efficiency.

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