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Free receptor recombinant adenovirus group for expressing porcine reproductive and respiratory syndrome virus (PRRSV), and preparation and application thereof

A technology for recombinant adenovirus and respiratory syndrome, applied in the direction of virus/bacteriophage, virus antigen components, biochemical equipment and methods, etc., to achieve a stable inhibitory effect

Inactive Publication Date: 2014-01-22
YANGZHOU UNIV
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  • Abstract
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Problems solved by technology

As for the CD163 free receptor and its blocking effect on PRRSV infection, there is no report yet

Method used

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  • Free receptor recombinant adenovirus group for expressing porcine reproductive and respiratory syndrome virus (PRRSV), and preparation and application thereof
  • Free receptor recombinant adenovirus group for expressing porcine reproductive and respiratory syndrome virus (PRRSV), and preparation and application thereof
  • Free receptor recombinant adenovirus group for expressing porcine reproductive and respiratory syndrome virus (PRRSV), and preparation and application thereof

Examples

Experimental program
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Embodiment Construction

[0029] Source of biological material:

[0030] 1. pShuttle-CMV vector: purchased from Stratagene, USA. Literature: He TC, Zhou S, da Costa LT, Yu J, Kinzler KW. et al. Proc Natl Acad Sci USA, 1998, 95(5):2509-2514.

[0031] 2. Escherichia coli BJ-5183-AD-1 strain: purchased from Stratagene Company of the United States. Literature: He TC, Zhou S, da Costa LT, Yu J, Kinzler KW. et al. Proc Natl Acad Sci USA, 1998, 95(5):2509-2514.

[0032] 3. AAV-293 cells: imported from the Cell Bank of the Chinese Academy of Sciences. Literature: Xie QW, Leung M, Fuortes M, Sassa S, Nathan C. Complementation analysis of mutants of nitric oxide synthase reveals that the active site requires two hemes. Proc Natl Acad Sci USA. 1996, 93(10):4891-4896 .

[0033] 4. pMD18-T vector: purchased from Takara Bioengineering (Dalian) Co., Ltd. (Takara Code: 6011).

[0034] 5. PRRSV reference strain VR2332: purchased from ATCC (ATCC VR2332) in the United States and preserved in our laboratory. literat...

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Abstract

The invention relates to a free receptor recombinant adenovirus group for expressing porcine reproductive and respiratory syndrome virus (PRRSV), and preparation and application thereof. The recombinant adenovirus group recombinant adenoviruses for expressing first four IgV structure fields of porcine sialic acid adhesin and porcine IgG Fc segment fusion proteins, and recombinant adenoviruses for expressing (5-9)th SRCR structure fields of virocapsid receptor CD163 and porcine IgG Fc segment fusion proteins. The recombinant adenovirus group is used for preparing a preparation for preventing and treating porcine reproductive and respiratory syndrome (PRRS).Compared with the existing vaccine, the recombinant adenovirus is replication-defective virus, can not be diffused and transmitted in the porcine body, and does not have the risk of toxicity diffusion and toxicity return; and the synergetic effect of the two expressed free receptors can obviously inhibiting PRRSV infection of different toxic strains, and can be developed as a novel preparation for preventing and controlling PRRS.

Description

technical field [0001] The invention relates to the fields of biotechnology and animal disease prevention and control, in particular to a preparation method and application of a recombinant adenovirus expressing a free receptor of porcine reproductive and respiratory syndrome virus. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease of pigs, characterized by reproductive failure in sows and respiratory symptoms in pigs of various ages. The disease first occurred in North America in 1987 and was introduced into my country in 1996, causing huge economic losses to the pig industry in the world. Porcine reproductive and respiratory syndrome virus (PRRSV) mainly infects the monocyte-macrophage system of pigs, leading to severe apoptosis and immunosuppression, and can cause persistent infection. In addition, the virus not only has multiple strains and rapid mutation, but also uses a variety of mechanisms to escape the...

Claims

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Application Information

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IPC IPC(8): C12N7/01A61K39/12A61K31/14
Inventor 孙怀昌陈阳郭睿张鑫宇夏晓莉
Owner YANGZHOU UNIV
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