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Method for fixing gastropod mollusk

A mollusk and fixing method technology, applied in the field of fixation of gastropod mollusks, can solve problems such as difficulty in collecting and fixing in the wild, and achieve the effect of avoiding corruption and preserving integrity

Inactive Publication Date: 2015-06-10
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problems such as difficulty in collecting and fixing molluscs such as gastropods and flapbranchs in the field, and provide a method for storing snails by first freezing and then fixing, which has the advantages of being simple and fast, and has the advantages of good fixing effect. A kind of immobilization method of gastropod mollusk

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Snails were collected in Nanji Island, Zhejiang Province. The samples were collected and cleaned and placed in an incubator with ice. The sample collection process is about 2 hours. After returning to the station, all the snails opened slightly. A toothpick was inserted between the health part and the shell, and the snail did not respond. After adding a fixative such as alcohol, the snail shrunk slightly, but was blocked by a toothpick, leaving a large hole for the fixative to enter the soft body. The fixed sample is easy to unscrew the soft part. The radula of snails is completely prepared, and the extraction effect of genomic DNA and mitochondrial DNA is good, which can meet the needs of PCR and other experiments.

Embodiment 2

[0020] Purchasing Paphia undulata in the Xiamen market, using a method similar to that of Example 1, the fixation effect of Paphelia undulata is good, which can fully meet the requirements of modern experiments.

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PUM

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Abstract

The invention relates to a gastropod mollusk, in particular to a method for fixing the gastropod mollusk. The method includes that biological samples of the mollusks like gastropods and lamellibranches are obtained, and surface impurities and sticky liquid are removed; the mollusks like gastropods and lamellibranches are placed in a heat insulating box with ice cubes at the bottom, a layer of material with poor heat conduction performance is arranged between the ice cubes and the biological samples for preventing the biological samples from contacting the ice cubes directly, and the heat insulating box stays dry; after low-temperature preservation, the mollusks like gastropods and lamellibranches basically lose contractility and react to the outside slowly, operculums expose or shells open slightly; foreign matters are inserted between operculums or double shells of the mollusks like gastropods and lamellibranches required to be fixed for preventing animals from closing the operculums or the shells under stimulation; fixing agent is added, animal killing and initial fixing process are completed,; according to experiment needs, the fixing agent is replaced regularly, and long-term complete conservation of the samples is achieved. The method is simple and quick, and fine in fixing effect.

Description

technical field [0001] The invention relates to a gastropod mollusc, in particular to a method for fixing the gastropod mollusc. Background technique [0002] Molluscs represented by snails are important intertidal organisms, and they have important scientific significance in terms of ecosystem function, morphological structure, active substances and genetic evolution. When conducting scientific research on snails, it is inevitable to collect samples and fix the snails. The most commonly used fixatives are alcohol and formaldehyde. The external shape of the snail is composed of the shell and the shell. During the fixation process, once the soft part of the snail is stimulated, it will be tightly retracted into the shell and the shell will be blocked by the shell. Ordinary fixatives cannot penetrate the shell and cutin and enter the soft body. Often, the snails are soaked in the fixative for a long time, and the internal soft tissue is partially corrupted and the shell is s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 丁少雄黄建荣李海柯才焕
Owner XIAMEN UNIV