Protein GhKT2 related to potassium ion absorption capacity of plant as well as coding gene and application thereof

A technology related to proteins and plants, which is applied to the protein GhKT2 related to the ability of plants to absorb potassium ions and its coding gene and application fields, which can solve the problems of low potassium absorption capacity and achieve the effect of improving low potassium tolerance

Active Publication Date: 2014-02-05
CHINA AGRI UNIV
View PDF7 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, cotton has a low ability to absorb potassium in the soil, so cot

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Protein GhKT2 related to potassium ion absorption capacity of plant as well as coding gene and application thereof
  • Protein GhKT2 related to potassium ion absorption capacity of plant as well as coding gene and application thereof
  • Protein GhKT2 related to potassium ion absorption capacity of plant as well as coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0037] Example 1. Discovery, expression analysis and subcellular localization of GhKT2 protein and its encoding gene

[0038] 1. The discovery of GhKT2 protein and its encoding gene

[0039] Use the database to search and compare the related gene sequences of other species, assemble and verify the EST sequence of cotton, perform 5'RACE and 3'RACE, and obtain a new protein from the cotton variety "Liaomian 17", which is named GhKT2 protein , as shown in Sequence 1 of the Sequence Listing (consisting of 792 amino acid residues). The gene encoding the GhKT2 protein is named GhKT2 gene, and the open reading frame of its cDNA is shown in sequence 2 of the sequence listing (consisting of 2379 nucleotides).

[0040] In order to analyze the differences between the GhKT2 gene and the homologous gene sequences of other species, the multiple sequence alignment structure of the EBP1 sequence was first constructed using clustalX version 1.83, and the default parameters of the software wer...

Example Embodiment

[0048] Example 2, the acquisition of transgenic plants

[0049] 1. Construction of recombinant plasmids

[0050] 1. The total RNA of the leaves of the cotton variety "Liaomian 17" was extracted and reverse transcribed into cDNA.

[0051] 2. Using the cDNA obtained in step 1 as a template, perform PCR amplification with a primer pair composed of F1 and R1 to obtain a PCR amplification product.

[0052] F1: 5'-GC TCTAGA ATGGATCTTGAGTTTGGGAAGT-3’

[0053] R1: 5'-C GAGCTC TTACACCACATAAACCATGCCA-3’

[0054] 3. The PCR amplification product obtained in step 2 was double digested with restriction endonucleases XbaI and SacI, and the digested product was recovered.

[0055] 4. The pBI121 vector was digested with restriction enzymes XbaI and SacI, and the vector backbone of about 12000bp was recovered.

[0056] 5. Connect the enzyme-digested product of step 3 with the vector backbone of step 4 to obtain a recombinant plasmid pBI121-GhKT2. The schematic diagram of the recombina...

Example Embodiment

[0074] Example 3. Identification of transgenic plants

[0075] MS Medium Recipe:

[0076] Macro elements: 1.65g NH 4 NO 3 , 1.9g KNO 3 , 0.17g KH 2 PO 4 , 0.37 g M g SO 4 ·7H 2 O. 0.44 gCaCl 2 ·2H 2 O;

[0077] Trace elements: 22.3mg MnSO 4 ·4H 2 O, 8.6 mg ZnSO 4 ·7H 2 O, 0.025mg CoCl 2 ·6H 2 O, 0.025mg CuSO 4 ·5H 2 O, 0.025mg Na 2 MoO 4 ·2H 2 O, 0.83 mg KI, 6.2 mg H 3 BO 3 ;

[0078] Iron salt: 27.8mg FeSO 4 ·7H 2 O, 37.3mg Na 2 EDTA;

[0079] Macroelements, trace elements, iron salts and 9 g of agar were dissolved in water and made up to 1 L with water to obtain MS medium (potassium ion concentration was about 19.9 mM).

[0080] Low potassium medium formula:

[0081] Macro elements: 2.3g NH 4 NO 3 , 0.37g MgSO 4 ·7H 2 O, 0.144g NH 4 H 2 PO 4 , 0.44g CaCl 2 ·2H 2 O;

[0082] Trace elements: the same as those in MS medium.

[0083] Iron salt: the same as the iron salt in MS medium.

[0084] Dissolve macroelements, trace elements, iro...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a protein GhKT2 related to potassium ion absorption capacity of a plant as well as a coding gene and application thereof. The protein provided by the invention is (a) or (b), wherein (a) is a protein composed of an amino acid sequence as shown in a sequence 1 in a sequence list, (b) is a protein formed by the way that substitution and/or deletion and/or addition of one or more than one amino acid residues are carried out on an amino acid sequence as shown in the sequence 1 and derivation related to low-potassium adversity stress resistance reaction of the plant, the potassium ion absorption capacity of the plant and development of the plant is carried out on the sequence 1. The invention discloses a new protein and gene, and after the gene is introduced into the plant, the low-potassium resistance of the plant can be obviously improved; the protein GhKT2 has a significant value on a new plant variety, especially a new cotton variety.

Description

technical field [0001] The invention relates to a protein GhKT2 related to the ability of plants to absorb potassium ions, its coding gene and application. Background technique [0002] Potassium is one of the three essential nutrients for plant growth, and is closely related to enzyme activity, protein synthesis, photosynthesis, oil synthesis, stomatal movement, ion balance and stress resistance. Potassium nutrient deficiency leads to slow plant growth, short plant and poor root development or even rot, and reduced stress resistance and disease resistance. [0003] In recent years, with the promotion of high-yield varieties in my country, the increase in the application of nitrogen and phosphorus fertilizers, and the improvement of the quality requirements of agricultural products, the scope of potassium-deficient soil in my country has gradually expanded. 1 / 4 to 1 / 3 of China's arable land is deficient in potassium or severely deficient in potassium. The potassium taken aw...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N5/10C12N1/15C12N1/19C12N1/21A01H5/00
CPCC07K14/415C12N15/8261C12N15/8271
Inventor 田晓莉徐娟
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap