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A kind of alkaloid compound and its preparation method and its application as tumor cell growth inhibitor

A technology for alkaloids and compounds, which is applied in the field of quinazoline alkaloid compounds and their preparation, can solve problems such as alkaloid-based selective tumor cell inhibitors and the like, and achieve the effect of broad application prospects.

Active Publication Date: 2016-03-30
OCEAN UNIV OF CHINA
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  • Abstract
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Problems solved by technology

However, in recent years, no alkaloid selective tumor cell inhibitors with important anti-tumor value have been obtained from marine microorganisms.

Method used

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  • A kind of alkaloid compound and its preparation method and its application as tumor cell growth inhibitor
  • A kind of alkaloid compound and its preparation method and its application as tumor cell growth inhibitor

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Embodiment Construction

[0010] Preparation of Compounds of the Invention

[0011] (1) Culture of endophytic fungi of Gorgonian coral

[0012] The medium used for the culture of the endophytic fungus Scopulariopsis sp. (TA01-33) of Gorgonian coral contains 1.0% glucose (weight percentage, the same below), 0.2% yeast extract, 0.2% peptone, 1.0% agar, and 3.0% sodium chloride , and the rest is water, which is made into a test tube slant when used, and the fungal strains are cultivated at 30°C for 5 days.

[0013] The strain culture medium contains glucose 0.1%-5.0% (weight percentage, the same below), yeast extract 0.01%-1%, peptone 0.01%-1%, agar 0.1%-3.0%, sodium chloride 0.05%- 5%, the rest is water; the culture temperature is 15-35°C, and the culture time is 3-10 days.

[0014] (2) Fermentation of gorgonian endophytic fungi

[0015] The fermentation medium used for the fermentation culture of the endophytic fungus Scopulariopsissp.(TA01-33) of Gorgonian coral contains 1.0% of glucose (percentage ...

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Abstract

The invention relates to a quinazoline alkaloid compound, a preparation method thereof and application of the quinazoline alkaloid compound as a tumor cell growth inhibitor. The preparation method comprises the following steps of: firstly culturing gorgonian endophytic fungi Scopulariopsis sp.(TA01-33) in a strain culture medium; then carrying out fermentation culture on the fungi in a fermentation culture medium; then filtering obtained fermentation liquor to remove thalli, decompressing and concentrating filter liquor, and extracting by using ethyl acetate; concentrating extract liquor, then carrying out normal-phase silicagel column chromatography and Sephadex LH-20 gel column chromatography, and concentrating obtained eluent to obtain a colorless crystal, namely fumiquinazoline L. The quinazoline alkaloid compound fumiquinazoline L obtained from marine fungi has selective inhibitory activity on a lung cancer cell A549, can be used for developing the tumor cell growth inhibitor and is wide in application prospect because raw materials can be produced on a large scale without being limited by resources.

Description

technical field [0001] The present invention relates to a quinazoline alkaloid compound with a novel seven-ring structure and its preparation method and application, in particular to a quinazoline alkaloid compound capable of selectively inhibiting the growth of lung cancer cell A549 and Its preparation method and application. Background technique [0002] Tumor is one of the major diseases affecting human health, and it is also the biggest medical problem faced by the world's medical community. It is urgently needed in the fields of medicine and pharmacy to find tumor cell growth inhibitors with good activity. The particularity of the marine environment endows the particularity of the metabolites of marine organisms. Searching for natural products with anti-tumor activity from marine organisms has become a research hotspot, especially the secondary metabolites of marine microorganisms have unique structures and significant activities, which have attracted Widespread concer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D498/22A61P35/00C12P17/18C12R1/645
CPCC07D498/22C12P17/188
Inventor 邵长伦王长云胥汝芳
Owner OCEAN UNIV OF CHINA
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