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Application of DJ-1 protein in preparation of product for diagnosis and therapy of osteosarcoma

A DJ-1, osteosarcoma technology, applied in the direction of peptide/protein components, preparations for in vivo tests, medical preparations containing active ingredients, etc., can solve problems such as functions that have not been fully proven

Inactive Publication Date: 2014-02-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the function of this protein has not yet been fully ascertained. Studies have reported that it is related to human autosomal recessive early-onset Parkinson's disease; in terms of tumors, it has been reported that DJ-1 is abnormally expressed in a variety of neoplastic lesions and may be involved in The occurrence and development of malignant tumors, but whether DJ-1 protein can be used as a marker molecule for the diagnosis of osteosarcoma and a therapeutic target has not yet been reported

Method used

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  • Application of DJ-1 protein in preparation of product for diagnosis and therapy of osteosarcoma
  • Application of DJ-1 protein in preparation of product for diagnosis and therapy of osteosarcoma
  • Application of DJ-1 protein in preparation of product for diagnosis and therapy of osteosarcoma

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Experimental program
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Effect test

Embodiment 1

[0023] A total of 51 clinical samples of osteosarcoma were selected, fixed with 10% formaldehyde solution for 7 days, and then paraffin-embedded tissue blocks were prepared. A paraffin section with a thickness of 8 microns was prepared from the sample, and the expression of DJ-1 protein (the amino acid sequence is shown in SEQ ID No: 1) was detected by immunohistochemical method, and at the same time, another section was selected for H&E staining. The expression of DJ-1 protein and H&E staining were observed under an upright microscope. see figure 1 , the expression of DJ-1 protein in the sample cancer tissue was higher than that in the non-tumor tissue in the same film.

Embodiment 2

[0025] According to statistics on the expression of DJ-1 protein in the 51 cases of osteosarcoma clinical samples in the aforementioned embodiment 1, it was found that 24 cases were strongly positive, 15 cases were positive, 7 cases were weakly positive, and 5 cases were negative ( figure 2 ).

[0026] The subcultured osteosarcoma cells KHOS and U2OS were transfected with DJ-1 gene (DJ-1 group), and a blank control (PCMV6 group) was set. After 24 hours of transfection, cisplatin, paclitaxel, doxorubicin and methotrexate were administered respectively, and the growth inhibitory effect of the drugs on the transfected cells was detected. see image 3 with Figure 4 , after overexpression of DJ-1 gene, osteosarcoma cells KHOS and U2OS were significantly less sensitive to drugs.

Embodiment 4

[0028] In subcultured osteosarcoma cells KHOS and U2OS, the DJ-1 gene was specifically knocked out by siRNA (SiRNA DJ-1 group), and a blank control (NC group) was set. After 24 hours of transfection, cisplatin, paclitaxel, doxorubicin and methotrexate were administered respectively, and the growth inhibitory effect of the drugs on the cells was detected. see Figure 5 with Image 6 , KHOS and U2OS osteosarcoma cells significantly increased drug sensitivity after knocking out the DJ-1 gene.

[0029] Zhejiang University

[0030] Application of DJ-1 protein in preparation of osteosarcoma diagnosis and treatment products

[0031] 7

[0032]

[0033] 1

[0034] 181

[0035] PRT

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Abstract

The invention provides application of a DJ-1 protein in preparation of products for diagnosis and therapy of osteosarcoma, and the amino acid sequence of the DJ-1 protein is shown as SEQID No:1. Experiments show that the expression of the DJ-1 protein in an osteosarcoma tissue is significantly higher than that in a non tumor tissue, and over expression (knockout) of the DJ-1 protein in osteosarcoma cells can significantly reduce (increase) the sensitivity of the osteosarcoma cells to first-line treatment drugs, so that the DJ-1 protein can be used as a specific marker protein for diagnosis and therapy of the osteosarcoma, and can improve the accuracy of the diagnosis of the osteosarcoma and the effectiveness of the therapy of the osteosarcoma. The DJ-1 protein provides a new therapeutic target and effective new drugs for the prevention and therapy of the osteosarcoma.

Description

Technical field [0001] The invention is the field of biopharmaceuticals, which involves the application of a DJ-1 protein in preparing diagnosis and the treatment of osteosarcoma products. Background technique [0002] Blumarousar is one of the most common malignant osteic tumors. Epigrheal studies have found that the disease occurs in young people aged 15-25, and men's incidence is higher than women.The good part of the osteosarcoma is a dry end of the long tube -shaped bone. The tumor is malignant and the prognosis is extremely poor. After the pure amputation, the 5 -year survival rate of patients is less than 20%.Therefore, effective diagnosis and prevention of osteosarcoma are the focus and difficulty in the current study of osteosoma. [0003] Human DJ-1 protein is encoded by the DJ-1 gene (GeneBank No.nm 007262.4) and is one of the members of the Thij / PFPI family.At present, the function of this protein has not been fully proven, and some research reports that it is related...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61K49/00A61P35/00
Inventor 杨波何俏军曹戟谢楠张俊
Owner ZHEJIANG UNIV
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