Detection method for health-care food functional components
A technology of health food and detection method, which is applied in the field of food analysis, can solve problems such as untreatable diseases, and achieve the effects of no complicated steps, easy popularization and application, and simple methods
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[0013] 1. Drawing the standard curve
[0014] Prepare the dextran standard stock solution, accurately weigh 0.5007g of the dextran reference substance dried to constant weight, add water to dissolve, and dilute to 50ml, mix well, and store in the refrigerator.
[0015] Prepare dextran standard use solution. Take 1.00ml of dextran standard stock solution, place it in a 100ml measuring flask, add water to the mark, mix well, and store in the refrigerator. This solution contains 0.10mg of dextran per 1ml.
[0016] Precisely draw dextran standard solution 0, 0.10, 0.20, 0.40, 0.60, 0.80, 1.00 ml (equivalent to dextran 0, 0.010, 0.020, 0.040, 0.060, 0.080, 0.10 mg) and place them in 25ml colorimetric tubes. Add water to 2.0ml accurately, add 1.0ml of 50g / L phenol solution, and mix on a rotary mixer. Carefully add 10.0ml of concentrated sulfuric acid and carefully mix on the rotary mixer until it boils in a boiling water bath for 2 minutes. After cooling to room temperature, use a spectr...
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