Multi-kernel support vector machine based dynamic fermentation process predicating method
A technology of support vector machine and fermentation process, which is applied in the field of support vector machine to establish a fermentation process model to predict biochemical parameters, and can solve the problem that the model cannot adapt to the new environment.
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[0048] Provide following embodiment in conjunction with content of the present invention:
[0049] The fermentation process of gene recombinant Escherichia coli to produce interleukin-2 is a typical multi-stage process, which mainly includes the stage of culture without feed, the stage of rapid growth of strain with feed, and the stage of inducing product synthesis. The whole fermentation cycle lasts about 19-20 hours, the sampling interval of the fermentation process is 0.5h, and the initial inoculation volume is 700ml.
[0050] The experimental data used in this experiment was obtained from a biopharmaceutical company in Yizhuang Economic Development Zone, Beijing. Using fed-batch fermentation, the actual fermentation process data of 5 batches of recombinant Escherichia coli exogenous protein expression to prepare interleukin-2 were obtained.
[0051] The specific implementation steps of the dynamic prediction method of the fermentation process product interleukin-2 realize...
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