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Nanometer gene transfer material, and preparation method and use thereof

A gene introduction and nanotechnology, applied in nanotechnology, nanomedicine, nanotechnology, etc., can solve the problems of unstable results, high toxicity, and limited penetration of the gene gun by calcium phosphate co-precipitation method, and achieve good biocompatibility. performance, low production cost and good dispersibility

Active Publication Date: 2014-03-12
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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Problems solved by technology

[0004] Among the several transfection methods of the above-mentioned non-viral gene introduction system, microinjection can only treat one cell at a time; the penetration of the gene gun is very limited; the result of the calcium phosphate co-precipitation method is unstable, and the transfection efficiency is affected by temperature, concentration, operation environment and many other factors; although the cationic liposome method has shown good transfection efficiency, its high toxicity limits its application

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  • Nanometer gene transfer material, and preparation method and use thereof
  • Nanometer gene transfer material, and preparation method and use thereof
  • Nanometer gene transfer material, and preparation method and use thereof

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Embodiment Construction

[0029] The present invention will be further described in conjunction with the following examples and accompanying drawings.

[0030] The nano gene transfer material of the present invention is made of C 18 V 2+ PtNPs–C with an average particle size of 100 nm prepared by reacting with chloroplatinic acid 18 V 2+ Composite, its preparation method is as follows:

[0031] 1. Preparation of materials:

[0032] Analytical pure C 18 V 2+ and chloroplatinic acid (product of Aldrich);

[0033] All glassware used for preparation were sonicated in ethanol for 5 minutes, then washed with double distilled water, and rinsed with washing solution H 2 0 / HNO 3 (Concentration 65%) / H 2 o 2 (concentration 35%) (1:1:1, v / v / v) for cleaning, followed by double-distilled water and acetone for cleaning, and finally air-dried.

[0034] 2. PtNPs–C 18 V 2+ Preparation of complex:

[0035] 1) Separately prepare C at a concentration of 6 mM with deionized water 18 V 2+ solution and a conce...

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Abstract

The invention relates to the technical field of gene transfer, and in particular relates to a nanometer gene transfer material, and a preparation method and use thereof. The nanometer gene transfer material is a PtNPs-C18V<2+> compound which is prepared by reacting C18V<2+> with chloroplatinic acid and has an average particle size of 100nm, and the nanometer gene transfer material is bonded with a green fluorescent protein gene pGFP in a non-covalent way to form an inorganic nanometer gene transfer system. As a non-viral transfer vector, the PtNPs-C18V<2+> compound shows high cell compatibility and is stable and high in transfer efficiency.

Description

technical field [0001] The invention relates to the technical field of gene introduction, in particular to a nanometer gene introduction material and its preparation method and application. Background technique [0002] With the completion of the determination of the human gene map, our understanding of the pathogenesis of human diseases has made breakthrough progress at the genetic level. Current research shows that the occurrence and development of many diseases are closely related to genes. If the gene fragments or gene mutations specifically related to the disease can be screened out, specific treatments can be targeted at the gene level, such as by introducing related deletion genes or silencing genes to enhance related deletion functions or silence disease-causing genes , so as to achieve the purpose of complete treatment. Safely and effectively introducing target genes into organisms is the key and difficult point in this research field. [0003] The gene transfer ...

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Application Information

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IPC IPC(8): C12N15/63B82Y5/00A61K47/48A61P35/00
Inventor 王深明徐安武张德元常光其周鸿雁李梓伦夏浩明张红霞
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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