Method for preparing biological active peptide with effect of reducing trioxypurine by using walnut protein

A technology of biologically active peptides and walnut meal, which is applied in the preparation method of peptides, chemical instruments and methods, medical preparations containing active ingredients, etc., can solve the problems of color and smell deterioration, limit the application field, etc., and achieve production costs Low, lower blood creatinine level, significant effect of lowering uric acid

Active Publication Date: 2014-03-12
广东华肽生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Walnut meal is the residue left after the walnut oil is squeezed. It contains more than 30% protein, 18 kinds of amino acids are complete, and the content of arginine and glutamic acid in the 8 kinds of essential amino acids is relatively high. However, due to its color and smell Deterioration during processing limits the application field. At present, most manufacturers sell it as feed raw material at a low price

Method used

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  • Method for preparing biological active peptide with effect of reducing trioxypurine by using walnut protein
  • Method for preparing biological active peptide with effect of reducing trioxypurine by using walnut protein
  • Method for preparing biological active peptide with effect of reducing trioxypurine by using walnut protein

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Embodiment 1

[0052] A method for preparing bioactive peptides with uric acid-lowering efficacy with walnut dregs, comprising the following steps:

[0053] (1) Pretreatment of walnut meal: add water to make the mass content of walnut meal reach 10% (w / w), heat at 100°C for 45 minutes, and pass through a colloid mill to obtain a walnut meal slurry.

[0054] (2) Restricted enzymatic hydrolysis: heat the walnut pulp slurry to 50°C, add 0.6% alkaline protease and 1.0% cellulase by walnut pulp mass, and inactivate the enzyme when the degree of hydrolysis reaches 10% (heat to Keep at 95-100°C for 15-30min), centrifuge, and take the supernatant, which is the enzymatic hydrolysis solution of walnut meal;

[0055] (3) Membrane separation-macroporous resin combination separation and enrichment of uric acid-lowering peptide: pass the walnut meal enzymatic hydrolysis solution through an ultrafiltration membrane with a membrane flux of 10,000Da, collect the permeate, and then pass it through a 001×7 gel...

Embodiment 2

[0060] A method for preparing bioactive peptides with uric acid-lowering efficacy with walnut dregs, comprising the following steps:

[0061] (1) Pretreatment of walnut meal: Add water to make the mass content of walnut meal reach 12% (w / w), heat at 110°C for 40 minutes, and pass through a colloid mill to obtain a walnut meal slurry.

[0062] (2) Restricted enzymatic hydrolysis: heat the walnut pulp slurry to 55°C, add alkaline protease of 0.8% by mass of walnut pulp and cellulase of 1.5% by mass of walnut pulp, and inactivate the enzyme when the degree of hydrolysis reaches 12% (heat to Keep at 95-100°C for 15-30min), centrifuge, and take the supernatant, which is the enzymatic hydrolysis solution of walnut meal;

[0063] (3) Membrane separation-macroporous resin combination separation and enrichment of uric acid-lowering peptide: pass the walnut meal enzymatic hydrolysis solution through an ultrafiltration membrane with a membrane flux of 10,000Da, collect the permeate, and ...

Embodiment 3

[0068] A method for preparing bioactive peptides with uric acid-lowering efficacy with walnut dregs, comprising the following steps:

[0069] (1) Pretreatment of walnut meal: add water to make the mass content of walnut meal reach 15% (w / w), heat at 100°C for 45 minutes, and pass through a colloid mill to obtain a walnut meal slurry.

[0070] (2) Restricted enzymatic hydrolysis: heat the walnut pulp slurry to 50°C, add 1.2% alkaline protease and 1.2% cellulase of walnut pulp, and inactivate the enzyme when the degree of hydrolysis reaches 15% (heat to Keep at 95-100°C for 15-30min), centrifuge, and take the supernatant, which is the enzymatic hydrolysis solution of walnut meal;

[0071] (3) Membrane separation-macroporous resin combination separation and enrichment of uric acid-lowering peptide: pass the walnut meal enzymatic hydrolysis solution through an ultrafiltration membrane with a membrane flux of 10,000Da, collect the permeate, and then pass it through a 001×7 gel-type...

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Abstract

The invention discloses a method for preparing biological active peptide with an effect of reducing trioxypurine by using walnut protein. The method comprises the following steps: watering and grinding the walnut protein; adding alkaline protease and cellulose; deactivating enzyme and centrifuging when the degree of hydrolysis achieves 10-15%, so as to obtain walnut protein enzymatic hydrolysate; filtering the enzymatic hydrolysate by virtue of an ultrafiltration membrane and strong acid gel type cation exchange resin, so as to obtain the biological active peptide with the effect of reducing trioxypurine. The biological active peptide is prepared by combining biological enzymolysis with a chromatographic separation technology; the target active peptide has a significant effect of reducing trioxypurine. An animal experiment in rats proves that the peptide for reducing trioxypurine prepared by the method disclosed by the invention can be used for significantly reducing the serum creatinine level of the rat, and has a certain degree of protective effect on the renal function of the rat, and strong activity of reducing the trioxypurine.

Description

technical field [0001] The invention relates to a method for preparing bioactive peptides with uric acid-lowering effect by using walnut dregs. Background technique [0002] Hyperuricemia refers to the supersaturated state of urate in the extracellular fluid. Hyperuricemia should be considered when the blood urate of men is ≥416umol / L and that of women is ≥357umol / L. [0003] Hyperuricemia is divided into two categories: primary and secondary. Primary hyperuricemia is caused by increased uric acid production, decreased excretion, or both. Gout is a clinical syndrome caused by urate crystal deposition. It is characterized by hyperuricemia, manifested as recurrent arthritis, tophi formation, and joint deformities. Involvement of the kidneys can cause chronic interstitial nephritis and uric acid. Nephrolithiasis, severe cases may cause joint disability and renal insufficiency. Therefore, uric acid-lowering treatment is very necessary. [0004] At present, drugs for reducing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K2/00C07K1/36C07K1/18C07K1/34A61K38/02A61P19/06
Inventor 苏国万赵谋明赵强忠崔春孙为正
Owner 广东华肽生物科技有限公司
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