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A microelectrode method for detecting calcification rate of coral larvae

A technology of calcification rate and microelectrode, which is applied in the direction of measuring devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of larval physiological indicators such as photosynthesis and calcification that no one has studied, so as to reduce the loss of sample volume, Improve detection efficiency and rapid detection effect

Inactive Publication Date: 2015-08-19
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although Chinese researchers have studied and analyzed the oocyte development of Staghorn coral (Li Yuanchao et al. 2007), and observed the spawning process and larval development process of Swelling Rose coral and Staghorn coral with microscopic microscope (Huang Jieying et al. 2011), Zhang Chenglong et al. (2010) used the buoyancy weighing method to calculate the calcification rate of adult corals, but no one studied the physiological indicators of larvae such as photosynthesis and calcification

Method used

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  • A microelectrode method for detecting calcification rate of coral larvae
  • A microelectrode method for detecting calcification rate of coral larvae
  • A microelectrode method for detecting calcification rate of coral larvae

Examples

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Embodiment 1

[0027] The microelectrode of the present embodiment detects the method for the calcification rate of coral larvae, and its specific steps are as follows:

[0028] (1) In July 2013, at the depth of 2 to 5m in the Sanya Luhuitou coral reef area, we collected normal-growing, healthy Pocillopora damicornis that had not yet released their larvae, brought them back to the laboratory, and then Put it into a 15L culture container, wait for the corals to release oval or round floating larvae, collect the floating larvae, after the floating larvae sink to the bottom, let the larvae attach to the plastic petri dish for about 15 days;

[0029] (2) After the larvae are attached to the plastic petri dish, take out the petri dish, and use a scalpel to cut the plastic petri dish into 1cm around the larvae 2 small pieces, taking care not to touch the larvae.

[0030] (3) After cleaning the 5ml micro-breathing bottle with 10% (v / v) dilute hydrochloric acid, wash it with distilled water for 3 t...

Embodiment 2

[0049] The microelectrode of the present embodiment detects the method for the calcification rate of coral larvae, and its specific steps are as follows:

[0050] (1) In April 2013, at a water depth of 2 to 5m in the Sanya Luhuitou coral reef area, we collected staghorn corals (Acropora sp.) that were growing normally, healthy and had not yet released their larvae, brought them back to the laboratory, and put them in Put it into a 15L culture container, wait for the corals to release oval or round swell larvae, collect the swell larvae, after the swell larvae sink to the bottom, let the larvae attach to the plastic culture plate for about 2 months;

[0051] (2) Take out the culture plate and use a scalpel blade to separate the larvae from the bottom.

[0052] (3) After cleaning the 5ml micro-breathing bottle with 10% (v / v) dilute hydrochloric acid, wash it with distilled water for 3 times;

[0053] (4) Put the larvae into a micro-breathing bottle and pour seawater through a m...

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Abstract

The invention discloses a method for detecting the coral larva calcification rate by means of microelectrodes. The method comprises the steps of collecting adult corals, placing the collected adult corals in an incubator, collecting planulae released by the corals to enable the planulae to be attached to an attachment, placing the planulae into a micro respirator bottle filled with sea water, conducting illumination / darkness cultivation for T1 / T2h and measuring the change of oxygen in the sea water continuously with an oxygen microelectrode and the change of the PH value of the sea water with a PH microelectrode at the same time, collecting data, comparing the data with data acquired from the same treatment on sea water not containing planulae, measuring the initial alkalinity before illumination / darkness cultivation, calculating photosynthesis and respiration of larvae according to the change of oxygen, calculating the change of the PH value caused by photosynthesis and respiration according to a carbonate balance system model CO2SYS, and obtaining the illumination calcification rate and darkness calcification rate of the coral larvae through calculation, wherein the T1h and T2h are between 0h and 12h.

Description

Technical field: [0001] The invention belongs to the field of coral calcification research, and in particular relates to a method for detecting the calcification rate of coral larvae with a microelectrode. Background technique: [0002] Coral reefs and related ecosystems have important ecological functions. They provide places for many marine organisms to lay eggs, reproduce, inhabit and avoid predators, and have important functions such as protecting coastlines and eco-tourism. Due to climate change, socio-economic development, and pressure from human activities, the coverage of living corals is rapidly decreasing, and the biodiversity of coral reefs is declining. If the coral larvae can be successfully obtained and the physiological indicators of the coral larvae can be effectively monitored, it will help to identify the main factors affecting the growth of coral larvae and promote the restoration of the coral reef ecosystem. [0003] Due to the difficulty of collecting, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033G01N27/00
Inventor 袁翔城李秀保黄晖袁涛张浴阳江雷
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI