Chloramphenicol immunoaffinity gel detection column

A technology for gel detection and chloramphenicol, applied in the fields of enzymology, analytical chemistry, and immunology, can solve problems such as limitations and test strip influence

Inactive Publication Date: 2014-03-26
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
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  • Application Information

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Problems solved by technology

However, the detection of test strips is also susceptible to the influence of

Method used

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  • Chloramphenicol immunoaffinity gel detection column

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Embodiment Construction

[0030] Below in conjunction with embodiment, the present invention is further described.

[0031] The invention is formed by immobilizing the antibody of chloramphenicol on the agarose gel and then packing it into a column. Utilizing the high specificity of the antigen-antibody reaction, when the sample solution passes through the immunoaffinity gel detection column, chloramphenicol is specifically bound to the column, and after adding the enzyme label, chloramphenicol competes with the enzyme label to bind to chloramphenicol on the antibody, and then add the substrate to develop the color. Its specific embodiment is:

[0032] (1) Preparation of chloramphenicol immunoaffinity gel detection column quality control layer and detection layer:

[0033] ① The quality control layer of the chloramphenicol immunoaffinity gel detection column is made of HRP antibody gel and blocking gel mixed at a ratio of 1:100. Take 150 μL of the mixed gel and add it to a 1 mL solid phase extraction...

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Abstract

The invention relates to an immunoaffinity gel detection column for visually fast detecting residual chloramphenicol in food. The chloramphenicol immunoaffinity gel detection column belongs to the field of immunology, enzymology and analytic chemistry. Cyanogen bromide-activated agarose gel is respectively coupled with a chloramphenicol antibody and a hydrogen peroxidase (HRP) antibody to prepare chloramphenicol antibody glue and HRP antibody glue; the cyanogen bromide-activated agarose gel is confined by confining liquid to prepare confining glue. The confining glue and the chloramphenicol antibody glue are mixed to prepare a detection layer, the confining glue and the HRP antibody glue are mixed to prepare a quality control layer, the quality control layer is filled in a 1ml solid phase extraction column, the working conditions of all steps in the detection process can be determined, and a novel immunoaffinity gel detection column for fast qualitatively and semi-quantitatively detecting residual chloramphenicol in food can be researched, with the detection limit of 1mug/L. When the product detects the residual chloramphenicol in food samples, no organic solvents and complicated pretreatment, as well as the assistance of large instruments are not required, the chloramphenicol immunoaffinity gel detection column has good usability and accuracy, and can meet the requirement for visual fast detection.

Description

technical field [0001] The invention belongs to the fields of immunology, enzymology and analytical chemistry, and in particular relates to the preparation of a chloramphenicol immunoaffinity gel detection column. Background technique [0002] Chloramphenicol is a broad-spectrum antibiotic that has a good inhibitory effect on both Gram-positive and Gram-negative bacteria. It binds to the A unit on the 50s subunit of the ribosome, hinders the transpeptide reaction of peptide phthalide-based transferase, and prevents the extension of the peptide chain, thereby achieving the purpose of inhibiting bacterial protein synthesis. Because of its high efficiency and low price, it has been widely used in animal husbandry to treat various infectious diseases of animals. But it has strong toxic and side effects. Research institutes at home and abroad have found that chloramphenicol residues in food exceed the standard, which can easily cause multiple hazards such as thrombocytopenia, ag...

Claims

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Application Information

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IPC IPC(8): G01N33/53
CPCG01N33/559G01N33/54306
Inventor 王硕生威袁萌刘津涛王俊平张燕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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