Immune hemolytic anemia detection kit and detection method thereof

Abstract

The invention discloses an immune hemolytic anemia detection kit, and also discloses a detection method using the kit. When the result of a direct antiglobulin test is positive and the result of an antibody screening test for antibody release liquid is negative, medicament-induced hemolytic anemia is determined; when the result of the direct antiglobulin test is positive, the result of a serum / plasma antibody screening test is positive and the result of the antibody screening test for antibody release liquid is negative, autoimmune hemolytic anemia (including hemolytic transfusion reaction) is determined. The types of the immune hemolytic anemia are diagnosed accurately according to the results so as to guide to correctly select appropriate medicaments for treating the patient in clinic. The method has high accuracy and is applicable to the detection by a test tube method or a microcolumn gel card method.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a detection kit for immune hemolytic anemia, and also relates to a method for using the kit to detect immune hemolytic anemia. Background technique [0002] With the large increase in the variety of drugs, especially the large-scale use of antibiotics in clinical practice, the cases of drug-induced hemolytic anemia have increased significantly. Due to immune factors, a large number of red blood cells have been destroyed, and hemolysis such as anemia, jaundice, and soy sauce-colored urine have occurred clinically. Symptoms, ranging from affecting the treatment effect, seriously delaying the treatment and endangering the life safety of patients. [0003] How to avoid patients with immune hemolytic anemia caused by the use of drugs, it is necessary to carry out timely and accurate detection of immune hemolytic anemia in patients, stop using drugs that can cause immune hemolytic a...

AI Technical Summary

Problems solved by technology

The reason is that the detection of drug-induced hemolytic anemia belongs to the scope of immunohematology detection, while the immunohematology detection is only carried out in the blood transfusion department or blood bank of the clinical hospital. Therefore, patients with drug-induced hemolytic anemia cannot be detected and diagnosed in time, resulting in a fatality rate of more than 30%.

[0005] Drugs sometimes cause the formation of antibodies against the drug itself, and the formed drug antibodies can be sensitized or adsorbed on the patient's red blood cells, resulting in a positive test of the patient's red blood cells for direct antihuman globulin , causing the destruction of red blood cells in patients, resulting in immune hemolysis, and the decrease of hemoglobin in patients

The results are: 1. The efficacy of the drugs used to treat patients is reduced or inhibited

2. Patients with anemia due to drug-induced hemolysis

[0007] At present, there is no detection kit for the detection of immune hemolytic anemia, and the content of the detection is limited to the direct antiglobulin test and the screening of unexpected antibodies in the patient's plasma. It is immune hemolytic anemia, the classification of immune hemolytic anemia and the cause of immune hemolytic anemia cannot be detected