Immune hemolytic anemia detection kit and detection method thereof

A technology of hemolytic anemia and detection kit, which is applied in the direction of biological testing, measuring devices, material inspection products, etc., and can solve the problems of inhibition, lethality, and failure to detect the reasons for the classification of immune hemolytic anemia

Active Publication Date: 2014-03-26
江苏中济万泰生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason is that the detection of drug-induced hemolytic anemia belongs to the scope of immunohematology detection, while the immunohematology detection is only carried out in the blood transfusion department or blood bank of the clinical hospital. Therefore, patients with drug-induced hemolytic anemia cannot be detected and diagnosed in time, resulting in a fatality rate of more than 30%.
[0005] Drugs sometimes cause the formation of antibodies against the drug itself, and the formed drug antibodies can be sensitized or adsorbed on the patient's red blood cells, resulting in a positive test of the patient's red blood cells for direct antihuman globulin , causing the destruction of red blood cells in patients, resulting in immune hemolysis, and the decrease of hemoglobin in patients
The results are: 1. The efficacy of the drugs used to treat patients is reduced or inhibited
2. Patients with anemia due to drug-induced hemolysis
[0007] At present, there is no detection kit for the detection of immune hemolytic anemia, and the content of the detection is limited to the direct antiglobulin test and the screening of unexpected antibodies in the patient's plasma. It is immune hemolytic anemia, the classification of immune hemolytic anemia and the cause of immune hemolytic anemia cannot be detected

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The present invention detects immune hemolytic anemia (test tube method) including:

[0027] 1. Direct anti-human globulin reagent: including anti-human globulin reagent (anti-IgG), anti-human globulin reagent (anti-C 3 d).

[0028] 2. Antibody release reagent (glycine-hydrochloride / ethylenediaminetetraacetic acid disodium disodium technology): including antibody release solution I, antibody release solution II, and antibody release solution III.

[0029] 3. Antibody screening reagent: broad-spectrum anti-human globulin reagent (anti-IgG+ C 3 d).

[0030] Detection method:

[0031] 1. Direct anti-human globulin test:

[0032] (1) The packed red blood cells of the fresh blood sample of the sample to be tested are washed once with physiological sodium chloride solution to obtain packed red blood cells, and the red blood cell suspension with a concentration of 2 to 4% is prepared with physiological sodium chloride solution.

[0033] (2) Take 3 test tubes and label them as "IgG" and "C...

Embodiment 2

[0052] The present invention detects immune hemolytic anemia (microcolumn gel method) including:

[0053] 1. Direct anti-human globulin reagent: microcolumn gel card includes anti-human globulin reagent (anti-IgG) gel column, anti-human globulin reagent (anti-C 3 d) Gel column, blank gel tube.

[0054] 2. Antibody release reagents: including antibody release solution I, antibody release solution II, and antibody release solution III.

[0055] 3. Antibody screening reagents: Microcolumn gel cards include broad-spectrum anti-human globulin reagents (anti-IgG+ C 3 d) Gel column.

[0056] Detection method:

[0057] 1. Direct anti-human globulin test:

[0058] (1) The packed red blood cells of the patient's fresh blood sample are washed once with physiological sodium chloride solution to obtain packed red blood cells, and the red blood cell suspension with a concentration of 0.5 to 1% is prepared with physiological sodium chloride solution.

[0059] (2) Take the direct anti-human globulin test...

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PUM

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Abstract

The invention discloses an immune hemolytic anemia detection kit, and also discloses a detection method using the kit. When the result of a direct antiglobulin test is positive and the result of an antibody screening test for antibody release liquid is negative, medicament-induced hemolytic anemia is determined; when the result of the direct antiglobulin test is positive, the result of a serum/plasma antibody screening test is positive and the result of the antibody screening test for antibody release liquid is negative, autoimmune hemolytic anemia (including hemolytic transfusion reaction) is determined. The types of the immune hemolytic anemia are diagnosed accurately according to the results so as to guide to correctly select appropriate medicaments for treating the patient in clinic. The method has high accuracy and is applicable to the detection by a test tube method or a microcolumn gel card method.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a detection kit for immune hemolytic anemia, and also relates to a method for using the kit to detect immune hemolytic anemia. Background technique [0002] With the large increase in the variety of drugs, especially the large-scale use of antibiotics in clinical practice, the cases of drug-induced hemolytic anemia have increased significantly. Due to immune factors, a large number of red blood cells have been destroyed, and hemolysis such as anemia, jaundice, and soy sauce-colored urine have occurred clinically. Symptoms, ranging from affecting the treatment effect, seriously delaying the treatment and endangering the life safety of patients. [0003] How to avoid patients with immune hemolytic anemia caused by the use of drugs, it is necessary to carry out timely and accurate detection of immune hemolytic anemia in patients, stop using drugs that can cause immune hemolytic a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/564G01N2800/22
Inventor 周珍祯陈芳芳
Owner 江苏中济万泰生物医药有限公司
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