Method for detecting anaplasma phagocytophilum employing loop-mediated isothermal amplification (LAMP) technology
A phagocytic and technical detection technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of low detection rate and cumbersome operation of detection methods, and achieve simple result judgment, simple operation and sensitivity. high effect
Inactive Publication Date: 2014-04-02
HENAN AGRICULTURAL UNIVERSITY
View PDF1 Cites 5 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0008] Aiming at the problems of low detection rate and cumbersome operation of the detection methods in the prior art, the present invention provides a method for detect...
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0032] The present invention is described in further detail below by embodiment.
[0033] The reagents are all commercially available, and the DNA templates used are all preserved in the applicant's laboratory.
[0034] Main reagents: Bst DNA polymerase large fragment, New England Biolab; Betaine, Sigma; Tap DNA polymerase, deoxynucleoside triphosphate (dNTP Mixture), DL2000DNA Marker , 6×Loading buffer, TaKaRa Biosynthesis Company; DNAGreen, TIANDZ; SYBR Green I, Solarbio; Genomic DNA Rapid Extraction Kit (blood), Shanghai Sangon Biosynthesis Co., Ltd.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more PUM
Login to view more Abstract
The invention discloses a method for detecting anaplasma phagocytophilum employing a loop-mediated isothermal amplification (LAMP) technology. The detection method comprises an LAMP primer design, building of an LAMP reaction system and adoption of an LAMP detection method. Specificity detection, sensitivity detection and fluorescent visual detection are adopted in the LAMP detection method. The anaplasma phagocytophilum 16SrRNA is taken as a target gene locus, and has the characteristics of being fast, sensitive, efficient, low in cost and the like.
Description
technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a method for detecting phagocytophilic aplasma by using a loop-mediated isothermal amplification technique (LAMP). Background technique [0002] Anaplasma phagocytophilum is an important zoonotic pathogen belonging to the genus Anaplasma in the family Anaplasmataceae of the order Rickettsiale. The pathogen is widely parasitic in granulocytes including humans, wild animals, domestic animals and rodents. Human granulocytic anaplasmosis (Human granulocytic anaplasmosis, HGA) is caused by A.phagocytophilum invading human peripheral blood neutrophils (Lj Zhang, Y Liu, et al. Nosocomial Transmission of Human Granulocytic Anaplasmosis in China [J]. The Journal of the American Medical Association, 2008, 300(19): 2263-2270). [0003] At present, the main detection methods of anplasma include etiological diagnosis, serological diagnosis and molecular biology diagnosis. [00...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more Application Information
Patent Timeline
Login to view more IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/04C12Q1/6844C12Q2531/119
Inventor 宁长申王金鸿菅复春吕亚莉张龙现王荣军张艳韩琼琼张文静
Owner HENAN AGRICULTURAL UNIVERSITY
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Try Eureka
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap