Method for extracting epiberberine and application thereof

A technology for epiberberine and its use, which is applied in the field of epiberberine extraction, can solve the problems of expensive raw materials, complex extraction technology of epiberberine, and insufficient research on the application of epiberberine, so as to protect the liver and Kidneys, prevention and treatment of cardiovascular diseases, effects of low-cost products

Active Publication Date: 2014-04-09
重庆伊士腾生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report about the hypolipidemic activity of epiberberine, the effect on diabetic complications, the protective effect on animal liver and kidney, and the effect on obesity and cardiovascular diseases.
[0004] In summary, although there are some reports on the extraction technology of epiberberine, the extraction technology of epiberberine is complicated, or the price of raw materials is relatively expensive, and the application research of epiberberine is more serious. Seriously insufficient

Method used

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  • Method for extracting epiberberine and application thereof
  • Method for extracting epiberberine and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 extracts epiberberine

[0032] After extracting the mother liquor of berberine or coptisine, adjust the pH of the solution to 5; concentrate under reduced pressure until the specific gravity of the solution is 1.01g / mL, after the solution is cooled to 10°C, add concentrated ammonia water until the concentration of ammonia water is 1mol / L; place to cool After 24 hours, filter, and the filtrate is put on the column at a rate of 0.1% of the column volume per minute (V / V) according to the ratio of solution to polyamide 1:0.1 (V / V); Wash the polyamide column with 1mol / L ammonia water at a rate of 0.1% per minute (V / V); then, wash it with a solution of 1mol / L ammonium chloride-ethanol (5:1) The epiberberine elution solution is obtained; the eluent is concentrated under reduced pressure and crystallized to obtain epiberberine; the epiberberine crystals are dried at 60° C. to obtain the finished product of epiberberine. The epiberberine that this method makes utilize...

Embodiment 2

[0033] Embodiment 2 extracts epiberberine

[0034] After extracting the mother liquor of berberine or coptisine, adjust the pH of the solution to 8; concentrate under reduced pressure until the specific gravity of the solution is 1.50g / mL, after the solution is cooled to 70°C, add concentrated ammonia water until the concentration of ammonia water is 1mol / L; place to cool After 24 hours, filter, and the filtrate is put on the column at a rate of 5% of the column volume per minute (V / V) according to the ratio of solution to polyamide 1:2 (V / V); after the completion of the column, use 5 times the resin volume Wash the polyamide column with 1mol / L ammonia water at a rate of 5% per minute (V / V); The epiberberine elution solution is obtained; the eluent is concentrated under reduced pressure and crystallized to obtain epiberberine; the epiberberine crystals are dried at 80° C. to obtain the finished product of epiberberine. The epiberberine that this method makes utilizes HPLC ana...

Embodiment 3

[0035] Embodiment 3 extracts epiberberine

[0036] After extracting the mother liquor of berberine or coptisine, adjust the pH of the solution to 7; concentrate under reduced pressure until the specific gravity of the solution is 1.25g / mL, after the solution is cooled to 40°C, add concentrated ammonia water until the concentration of ammonia water is 1mol / L; place to cool After 24 hours, filter, and the filtrate is put on the column at a rate of 0.5% of the column volume per minute (V / V) according to the ratio of solution to polyamide 1:0.5 (V / V); Wash the polyamide column with 1mol / L ammonia water at a rate of 1% per minute (V / V); then, wash with a solution of 1mol / L ammonium chloride-ethanol (5:1) (3 times the volume of the resin) The epiberberine elution solution is obtained; the eluent is concentrated under reduced pressure and crystallized to obtain epiberberine; the epiberberine crystals are dried at 70° C. to obtain the epiberberine finished product. The epiberberine t...

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Abstract

The invention belongs to the technical field of extraction of a traditional Chinese medicine, and particularly relates to a method for extracting epiberberine and application thereof. The invention aims at solving the technical problem of providing new use of the epiberberine. The invention provides use of the epiberberine in preparation of a lipid-lowering drug. The invention also discloses use of the epiberberine in preparation of a drug for preventing or treating obesity. The invention also provides use of the epiberberine in preparation of an antioxidant drug. The invention also provides use of the epiberberine in preparation of a drug for preventing or treating atherosclerosis. The invention also provides an extraction method of the epiberberine. The method can be applied to preparation of the lipid-lowering drug, the antioxidant drug, the drug for preventing or treating atherosclerosis, the drug for preventing or treating obesity, and the like.

Description

technical field [0001] The invention belongs to the technical field of extraction of traditional Chinese medicines, and in particular relates to a method and application of extracting epiberberine. Background technique [0002] Coptis chinensis was first recorded in "Shen Nong's Materia Medica" and listed as top grade. Coptis chinensis is bitter in taste, cold in nature, and has the functions of purging fire, detoxifying, clearing away heat, and drying dampness. The results of modern pharmaceutical research show that the main active ingredients of Coptis chinensis are Coptis chinensis alkaloids, mainly including berberine (berberine), palmatine, coptisine and epiberberine ("Chinese Pharmacopoeia" 2010 edition); It has a wide range of pharmacological activities such as lowering blood fat and blood sugar; recent studies have found that, in addition to berberine, other alkaloid active components of Coptidis Rhizome (coptisine, palmatine, etc.) also have the effect of lowering ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4375A61P3/06A61P3/04A61P3/10A61P9/10C07D455/03
Inventor 李学刚叶小利
Owner 重庆伊士腾生物科技有限公司
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