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Root canal-periapical complex in vitro model

A periapical, complex technology, used in bioreactor/fermenter combinations, biochemical instruments, biochemical equipment and methods, etc.

Inactive Publication Date: 2014-04-09
STOMATOLOGICAL HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the infected root canal and periapical infection (lesions) can only be studied separately

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The establishment of the root canal-periapical complex in vitro model of embodiment 1

[0034] (1) Take a single premolar extracted due to orthodontics, use a curette and an ultrasonic scaler to remove the soft tissue and calculus on the root surface, place it in 2.5-3.0mol / L formaldehyde solution, and store it at 20-30°C for later use , to obtain isolated teeth (1);

[0035] (2) Take multiple penicillin bottles. The penicillin bottle is composed of the bottle body (2) and the rubber stopper (3). Dig a hole in the center of the rubber stopper (3) according to the diameter of the neck of the isolated tooth, and place the isolated tooth (1) Put it into the rubber stopper (3) through the hole, and seal the interface between the isolated tooth (1) and the rubber stopper (3) with light-cured flowing resin, and fix the bottle body (2) and the part with the isolated tooth (1) The rubber stopper (3) is sterilized at a temperature of 110-125°C and a pressure of 10-20MPa;

[00...

Embodiment 2

[0038] Example 2 Establishment of root canal-periapical complex in vitro model

[0039] (1) Take a single premolar extracted due to orthodontics, use a curette and an ultrasonic scaler to remove the soft tissue and calculus on the root surface, place it in 2.5-3.0mol / L formaldehyde solution, and store it at 20-30°C for later use , to obtain isolated teeth (1);

[0040](2) Take multiple penicillin bottles. The penicillin bottle is composed of the bottle body (2) and the rubber stopper (3). Dig a hole in the center of the rubber stopper (3) according to the diameter of the neck of the isolated tooth, and place the isolated tooth (1) Put it into the rubber stopper (3) through the hole, and seal the interface between the isolated tooth (1) and the rubber stopper (3) with light-cured flowing resin, and fix the bottle body (2) and the part with the isolated tooth (1) The rubber stopper (3) is sterilized at a temperature of 110-125°C and a pressure of 10-20 MPa;

[0041] Randomly s...

Embodiment 3

[0045] Embodiment 3 simulates no source of infection outside the root canal

[0046] Fifteen in vitro models of the complex established in Example 1 or 2 were used, and 5 models were randomly selected on the first day to detect bacteria in the periapical tissue. If bacteria were detected, it indicated that the model preparation was unsuccessful, and the model was remade; if no bacteria were detected, the remaining 10 models were naturally placed in the air without any treatment, and periapical bacteria were detected on the 21st day. At the same time, two positive control groups were set up: the penicillin vial containing the culture medium was directly exposed to the air. Using polymerase chain reaction (PCR, the primers are 16SrDNA common primers shared by bacteria: upstream: 5`-AGAGTTTGATCCTGGCTCAG-3`; downstream: 5`-GGTTACCTTGTTACGACTT-3`, the fragment length is about 1500bp, provided by Shanghai Sangong Bioengineering Co., Ltd. Synthesis.) Detect the presence or absence o...

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Abstract

The invention aims at providing a root tube-periapical complex in vitro model. An extracted tooth of the complex in vitro model is consistent with the condition of a living body, and an LB solid medium (various biological related components can be added) is used for simulating periapical tissue. The complex in vitro model provided by the invention does not have a root canal outer infection source, can simulate the infection of a root canal under a natural state which is the same as the living body, namely the infected root canal, and can observe the invasion condition of infection to the periapical tissue, the influence of clinical operation on the invasion of the infection to the periapical tissue, and the influence of periapical antibacterial substances (immune substances) on the invasion of the infected root canal to the periapical tissue.

Description

technical field [0001] The invention belongs to the field of root canal and periapical models; more specifically, the invention relates to a root canal-periapical complex in vitro model. Background technique [0002] Bacteria are the main pathogenic factors of periapical periodontitis. Some scholars believe that the bacteria in the root canal exist in the form of biofilm. Without treatment, the bacteria are not easy to penetrate the apical foramen, but the apical periodonitis caused by their virulence factors. Some scholars have also proposed that under the induction of environmental factors, some planktonic bacteria in the root canal biofilm can penetrate the apical foramen and dentinal tubules and invade the periapical tissue to form a root biofilm. In addition to direct bacterial invasion of the periapical tissue, bacterial virulence factors such as endotoxin in the root canal can also enter the periapical tissue and cause damage to the periapical tissue. Based on the c...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12Q1/02
CPCC12Q1/02
Inventor 钟晓波何斌王容骆书美赵洁曹祥莉
Owner STOMATOLOGICAL HOSPITAL OF CHONGQING MEDICAL UNIV
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