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Method, kit and primer for detecting pathogenic bacteria of lotus fungal rot disease carried by lotus seeds

A fungal and spoilage technology, applied in the field of fungal spoilage pathogen detection of lotus root

Active Publication Date: 2015-07-15
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no reports or publications about the specific detection primers designed for the lotus-specific type of Fusarium oxysporum and the method of using them to detect whether the lotus root seeds carry the pathogenic bacteria of the lotus root spoilage disease.

Method used

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  • Method, kit and primer for detecting pathogenic bacteria of lotus fungal rot disease carried by lotus seeds
  • Method, kit and primer for detecting pathogenic bacteria of lotus fungal rot disease carried by lotus seeds
  • Method, kit and primer for detecting pathogenic bacteria of lotus fungal rot disease carried by lotus seeds

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Embodiment 1

[0036] A. Isolation of pathogenic bacteria for testing:

[0037] The pathogenic bacteria tested came from the pathogenic bacteria isolated from lotus root with diseased lotus root in Changsha County, Huangxing Town and Xiangtan County and other places where the lotus root rot disease occurred.

[0038] The separation steps are as follows:

[0039] (a), wash the diseased lotus root with clean water, and cut it into about 2mm with sterilized scissors 2 small pieces or flakes of

[0040] (b) Place in 75% alcohol for surface disinfection, then soak in 0.1% acidic mercuric acid aqueous solution for 3-5 minutes after 10 seconds, and then rinse with sterile water for 3 times;

[0041] (c), use sterilized absorbent paper to dry up the sterile water remaining on the surface of the lotus root block, and finally transfer it to PDA medium containing agricultural streptomycin (40 μg / ml), and culture at a constant temperature of 25°C;

[0042] (d), After 5 days, pick a small amount of hy...

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Abstract

The invention discloses a method, kit and primer for detecting the pathogenic bacteria of lotus fungal rot disease carried by lotus seeds. In the invention, by utilizing the molecular biological technology, the type of the pathogenic bacteria of lotus rot disease is effectively identified and determined to be a fusarium oxysporum lotus-specialized type; a pair of primers with high specificity on the pathogenic bacteria genome of lotus rot disease is designed according to the sequencing result of the pathogenic bacteria exclusively causing the lotus rot disease; in combination with the PCR (polymerase chain reaction) technology, a fast and high-sensitivity molecular detection technology of the pathogenic bacteria of lotus rot disease is established; the technology can perform bacterium-carrying detection on the lotus seeds to realize early diagnosis and prevention of the lotus rot disease, and is of great significance to the green control of lotus disease and pollution-free planting.

Description

technical field [0001] The invention belongs to the technical field of detection of fungal spoilage pathogens of lotus root, and in particular relates to a method, a kit and primers for detecting lotus root fungal spoilage pathogens. Background technique [0002] Lotus root (including lotus root and seed lotus) belongs to the water lily family, and is a perennial herbaceous plant widely planted at home and abroad. my country has a very long history of planting lotus root. The roots, stems and fruits of lotus root are rich in starch, protein and vitamins. They have high economic value and are an excellent aquatic vegetable. They have become one of the important crops for farmers' economic growth. In recent years, with the adjustment of the planting industry structure, the planting area of ​​lotus root in my country has increased significantly. It is estimated that the current planting area of ​​lotus root in China is about 330,000 hm 2 above. [0003] Lotus root rot is an i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11C12R1/77
CPCC12Q1/686C12Q2531/113
Inventor 魏林梁志怀张屹成燕清陈玉荣吕刚肖密
Owner HUNAN PLANT PROTECTION INST
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