Heart puncture blood collecting device and puncture blood collecting method thereof for mice of medical research experiments

Abstract

The invention discloses a heart puncture blood collecting device and a puncture blood collecting method thereof for mice of medical research experiments. The device comprises an operation plate, an anesthesia cup and a blood collecting needle, wherein the operation plate is an oblong flat plate; the middle part of the flat plate is provided with a concave groove; the front end of the concave groove is provided with a clamping groove; the interior of the clamping groove is provided with an anesthesia small tube; the left side of the concave groove is provided with a neck binding band; the back side of the neck binding band is provided with a breast binding band; the upper end of the neck binding band is provided with a neck binding upper touch and close fastener; the right side of the concave groove is provided with a neck binding lower touch and close fastener; the upper end of the breast binding band is provided with a breast binding upper touch and close fastener; the right side of the concave groove is provided with a breast binding lower touch and close fastener. The device has the advantages that the structure is simple, the design is skillful, the heart positioning is accurate, and the operation is easy, convenient and quick.

Description

technical field [0001] The invention relates to a medical research equipment, specifically a heart puncture and blood collection device for experimental rats. Background technique [0002] In medical research, people often use mice to make disease models, to observe the kinetics of drug metabolism in animals or to study and evaluate the effect of a certain treatment method. According to statistics, more than half of the scientific research topics in medical biology are completed with rodents. Rats are the most numerous experimental animals in medical research, and how to draw blood samples has become one of the most basic and important pre-procedure steps in scientific research. At present, most of the existing blood collection methods adopt tail clipping blood collection, retro-orbital venous plexus blood collection, decapitated blood collection, sublingual vein blood collection, penis dorsal vein blood collection, etc. These methods cannot meet the requirements of detecti...

AI Technical Summary

Problems solved by technology

However, the traditional cardiac puncture blood collection has the following disadvantages: (1) The mouse is easily awakened during the blood collection process with too shallow anesthesia, but the blood collection needle is still in the heart, so it will seriously damage the heart. Difficulty in blood collection or less blood collection, and even death due to overuse of anesthesia; (2) Rats lie on their backs on a wooden board and fixed with four rubber bands. The position of the heart deviates, which is not conducive to finding the heart; (3) the use of the No. 7 needle, because the needle is too long, it is easy to puncture the internal organs and other organs and cause greater damage to the mouse; (4) the position of the heart is not easy to determine, beginners It takes a long time to practice to find the exact position of the heart; (5) The strength and depth of the needle insertion are not easy to control. If the strength is too strong, it will pierce the heart and other organs. If the strength is not enough, it will not be able to penetrate the heart; Keeping constant and slow to draw blood requires the operator to have strong control and perseverance, and the operation is difficult

(7) Rats that have been blood drawn have a high mortality rate and cannot be used for follow-up scientific research

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