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Method for determining true nutritive value of small intestines on nutrients by adopting three-step in-vitro technique

A technology of small intestine digestibility and technology, which is applied in the direction of measuring devices, analysis materials, instruments, etc., can solve the problems that the influence of starch and fat cannot be eliminated, the nutritional value of feed cannot be fully evaluated, and the digestibility of starch and fat in the small intestine cannot be measured. Ease of realization, cost saving and simple operation

Inactive Publication Date: 2014-04-30
CHINTEM TECH CONSULTING BEIJING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method cannot measure the digestibility of starch and fat in the small intestine, cannot fully evaluate the nutritional value of a certain feed, and cannot eliminate the influence of starch and fat in the feed on protein digestibility

Method used

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  • Method for determining true nutritive value of small intestines on nutrients by adopting three-step in-vitro technique
  • Method for determining true nutritive value of small intestines on nutrients by adopting three-step in-vitro technique
  • Method for determining true nutritive value of small intestines on nutrients by adopting three-step in-vitro technique

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Embodiment Construction

[0023] The present invention will be further described in detail below in conjunction with the drawings and specific embodiments:

[0024] The present invention is a three-step in vitro method for determining the digestibility of nutrients in the small intestine. The specific steps are:

[0025] 1) Rumen culture

[0026] Weigh about 15g of feed, put it into a 9cm×15cm nylon bag, and hang it in the rumen for 16 hours. After incubation, rinse the bag with tap water until the running water is clear, and dry at 55°C for 48 hours. Take out the residual sample in the bag and measure N content, starch content, fat content;

[0027] 2), acid treatment

[0028] Weigh 0.6~0.7g of the residual sample, put it into a 50ml centrifuge tube, add 10ml of 0.1mol / L HCl solution with a pH of 1.9, and incubate with shaking in a water bath at 38°C for 1 hour;

[0029] 3) Pancreatic juice culture

[0030] Use pancreatic juice for culturing. After culturing is completed, immediately add 3ml of 100% (wt / vol) TCA...

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Abstract

The invention discloses a method for determining the true nutritive value of small intestines on nutrients by adopting a three-step in-vitro technique. The method comprises the following three steps of culturing rumens, carrying out acid treatment and culturing pancreatic juice. The three-step in-vitro technique provided by the invention can be used for changing the method for feed treatment by utilizing pancreatic enzymes in the prior art, is used for direct treatment by utilizing pancreatic juice, can be used for digesting and degrading RUP (rumen un-degradation feed protein), and also can provide a favorable way for the estimation of ruminant small intestine starch true nutritive value and fat true nutritive value by adopting the pancreatic juice method. By adopting the method, the digestion situation of the ruminant on protein, starch and fat in feed can be simulated once, and three important indexes of the protein, starch and fat small intestine true nutritive value can be determined, and the method is simple to operate, is easy to realize, and saves the cost.

Description

Technical field [0001] The invention relates to a method for measuring the digestibility of the small intestine of ruminants, in particular to a method for measuring the digestibility of nutrients in the small intestine by adopting a three-step in vitro technique of pancreatic juice. Background technique [0002] In the small intestine of ruminants, metabolizable protein is composed of microbial protein synthesized in the rumen, rumen non-degradable feed protein (RUP) and a small amount of endogenous protein. Studies have shown that the digestibility of microbial protein in the small intestine is basically fixed (80%-85%), but the digestibility of the RUP in the small intestine is affected by factors such as feed type, feed source and processing method. The digestibility of feed RUP in the small intestine is an important parameter that affects the milk production and performance of dairy cows. [0003] At present, the protein evaluation system uses fixed values ​​for the RUP small...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
Inventor 徐明高明
Owner CHINTEM TECH CONSULTING BEIJING CO LTD