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A method for improving the germination rate of wild Aconitum aconitum seeds

A technology for the germination rate of Aconitum aconitum, which is applied to the field of increasing the germination rate of Aconitum aconitum wild seeds, can solve the problems of low germination rate of Aconitum aconitum seeds, reproduction and cultivation, destruction of grassland ecological balance, resource shrinkage, etc. Achieve the effects of protecting germplasm and artificial cultivation, protecting the ecological environment, and simple methods

Inactive Publication Date: 2016-04-06
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in recent years, due to the over-excavation or predatory exploitation of the medicinal and economic value of Aconitum aconitum, it has faced the situation of gradually shrinking resources, grassland degradation, and destruction of grassland ecological balance. Therefore, strengthening the protection of wild medicinal plant resources Protection and reasonable and artificial domestication cultivation have become important research topics at present
Our research found that the low germination rate of wild Aconitum aconitum seeds has become the primary factor limiting its reproduction and cultivation. At present, most of the research on wild Aconitum aconitum focuses on active ingredients and pharmacological effects, while There are few research reports on the mechanism and method of breaking dormancy of wild Aconitum chinensis seeds
Gibberellin GA 3 As a plant hormone, it has the effect of breaking dormancy. There are related research reports in other plant and seed germination studies, but there are few studies on the dormancy mechanism and breaking method of wild Aconitum aconitum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for improving the germination rate of wild Aconitum vulgare seeds, including the following steps:

[0015] 1. The test material was wild Aconitum vulgare seeds, collected from the Zaoxi grassland in Tianzhu County, Gansu Province at an altitude of 2960-2970m. The plants of Aconitum vulgare were collected directly, and the seeds were air-dried and the husks were removed manually. Store in a low temperature and dry environment for later use;

[0016] 2. Select the same size and plump Aconitum seed, disinfect and sterilize it with 5% sodium hypochlorite solution for 5min, and then rinse twice with distilled water. 3 Soak in the solution for 24 hours, and then place it in a petri dish (φ9cm) with two layers of filter paper. Place 100 grains in each dish, and set three replicates for each concentration;

[0017] 3. Put all the petri dishes in two incubators, and cultivate them under 3000LX light and dark, 18℃(12h)~25(12h)℃ variable temperature conditions, with seed white a...

Embodiment 2

[0030] The specific implementation mode is the same as in Example 1, except that in step 2, disinfection and sterilization with 5% sodium hypochlorite solution for 10 minutes, and then rinse with distilled water 3 times.

Embodiment 3

[0032] The specific implementation mode is the same as in Example 1, except that in step 2, disinfection and sterilization with 5% sodium hypochlorite solution for 8 minutes, and then rinse with distilled water 3 times.

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PUM

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Abstract

The invention discloses a method for improving seed germination rate of wild aconitum gymnandrum maxim. The method comprises the steps of removing shells of seeds of the wild aconitum gymnandrum maxim, disinfecting, and soaking the seeds in 1000mg / L gibberellin solution for 24h; respectively carrying out variable temperature cultivation at 18-25 DEG C for 12h in the light environment and dark environment to promote seed germination. The method has the beneficial effects that plant hormone GA3 is used for treating the seeds of the wild aconitum gymnandrum maxim, so that in the light environment, the seed germination rate of the aconitum gymnandrum maxim is increased to 91.7% from 7%, and in the dark environment, the seed germination rate of the aconitum gymnandrum maxim is increased to 95.7%. The method has the advantages that the method is simple and economical, is not influenced by factors such as seasons, climate and natural disasters; after the technology is utilized, the problems that the seeds of the aconitum gymnandrum maxim are low in reproductive capacity and short of resource can be solved, the demand for the seeds of the aconitum gymnandrum maxim can be met by artificial domestication cultivation, and the method has important significance for protecting the natural resource and natural ecological environment of the wild aconitum gymnandrum maxim.

Description

Technical field [0001] The present invention relates to a method for improving the germination rate of seeds, in particular to a method for improving the germination rate of wild Aconitum seed. Background technique [0002] Aconitum gymnandrum Maxim. is the only species of Ranunculaceae (Ranunculaceae) Delphinieae (tribe Delphinieae) Aconitum (AconitumL.) subgen. Gymnaconitum (Stapf) Rapaics, only distributed in China -Himalayan plant subflora and neighboring areas, mainly distributed in Tibet, western Sichuan, Qinghai, and southern Gansu. It grows on mountain grass slopes, field grasslands and riverside sandy land between 1550-3800m above sea level. Aconitum vulgare is closely related to rare Chinese medicinal materials such as Chuanwu and Caowu. Its leaves, flowers and roots can be used as medicine. It has a pungent taste and warm nature. It has the effects of dispelling wind and calming, repelling insects and killing maggots, and can be used to treat joints. Pain, rheumatism ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/02
Inventor 魏小红曹丽龙雨刘文瑜赵萌许可成
Owner GANSU AGRI UNIV
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