A method for improving the germination rate of wild Aconitum aconitum seeds
A technology for the germination rate of Aconitum aconitum, which is applied to the field of increasing the germination rate of Aconitum aconitum wild seeds, can solve the problems of low germination rate of Aconitum aconitum seeds, reproduction and cultivation, destruction of grassland ecological balance, resource shrinkage, etc. Achieve the effects of protecting germplasm and artificial cultivation, protecting the ecological environment, and simple methods
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Embodiment 1
[0014] A method for improving the germination rate of wild Aconitum vulgare seeds, including the following steps:
[0015] 1. The test material was wild Aconitum vulgare seeds, collected from the Zaoxi grassland in Tianzhu County, Gansu Province at an altitude of 2960-2970m. The plants of Aconitum vulgare were collected directly, and the seeds were air-dried and the husks were removed manually. Store in a low temperature and dry environment for later use;
[0016] 2. Select the same size and plump Aconitum seed, disinfect and sterilize it with 5% sodium hypochlorite solution for 5min, and then rinse twice with distilled water. 3 Soak in the solution for 24 hours, and then place it in a petri dish (φ9cm) with two layers of filter paper. Place 100 grains in each dish, and set three replicates for each concentration;
[0017] 3. Put all the petri dishes in two incubators, and cultivate them under 3000LX light and dark, 18℃(12h)~25(12h)℃ variable temperature conditions, with seed white a...
Embodiment 2
[0030] The specific implementation mode is the same as in Example 1, except that in step 2, disinfection and sterilization with 5% sodium hypochlorite solution for 10 minutes, and then rinse with distilled water 3 times.
Embodiment 3
[0032] The specific implementation mode is the same as in Example 1, except that in step 2, disinfection and sterilization with 5% sodium hypochlorite solution for 8 minutes, and then rinse with distilled water 3 times.
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