The invention relates to the technical field of traditional Chinese medicine compositions for degerming and killing odontobutis, and relates to a traditional Chinese medicine composition for degermingand killing tooth worms and a preparation method and an application thereof. The traditional Chinese medicine composition comprises the raw materials of corydalis bungeana, rhubarb, subprostrate sophora, berberis japonica, amaranth, pterocephalus hookeri, gentiana glabra, shepherd's purse, gypsum, aconitum gymnandrum, sweet wormwood, caryopteris clandonensis, tuber phlomis umbrosa, liquorice, common carpesium fruit, pepper, alpine cress, euphorbia, pumpkin seeds, polygonum tuberosum and Triosteum pinnatifidum. The gypsum is soaked in an extracting solution of 20 traditional Chinese medicinalmaterials which are decocted and concentrated and then dried to obtain the traditional Chinese medicine composition. The preparation method is simple, and the medicinal materials supplement each otherand play a synergistic role, so that the traditional Chinese medicine composition has the effects of clearing away heat and toxic materials, diminishing inflammation, sterilizing, dispelling cold, relieving pain, washing teeth and killing tooth worms, has a remarkable curative effect on toothache caused by tooth bacteria and the tooth worms, is not easy to relapse and can also be used for preventing breeding of the tooth bacteria and the tooth worms.