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A kind of preparation method of lutein

A technology of lutein and dried flowers, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of large amount of organic solvent, complicated process, long time consumption, etc. low effect

Inactive Publication Date: 2015-12-16
HARBIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing methods for preparing lutein have the disadvantages of large amount of organic solvent, complicated process, and long time consumption.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0026] A preparation method of lutein, characterized in that: using dried marigold flower granules as raw material, comprising the following steps:

[0027] (1) Put the dried marigold flower granules in the extraction tube of the Soxhlet extractor, add petroleum ether, ethanol, and potassium hydroxide in sequence in the extraction bottle of the Soxhlet extractor, and conduct the extraction under the conditions of heating, stirring, and avoiding light. extraction-saponification;

[0028] (2) Filter the mixture in the extraction bottle of the Soxhlet extractor, collect the filter residue, wash the filter residue with an organic solvent until the filtrate is colorless, collect the filtrate, and distill under reduced pressure at 30~55°C to extract 60~50% of the total weight of the organic solvent. 85%, put the concentrated liquid at a temperature of -5~-10°C, let it stand for 15~20 hours, collect the precipitated solid, and dry it in vacuum to obtain the lutein product.

[0029] ...

example 1

[0032] Put 100 grams of marigold dried flower granules into the extraction tube (volume 150 ml) of the Soxhlet extractor, add 180 ml of petroleum ether with a boiling range of 30-60 ° C, 1500 ml Ethanol aqueous solution with a volume concentration of 80%, 0.6 g of potassium hydroxide, extraction-saponification for 12 hours at a heating temperature of 65°C, stirring, and dark conditions; after extraction-saponification, filter the extraction bottle of the Soxhlet extractor The mixture in the mixture, collect the filter residue, wash the filter residue with ethyl acetate until the filtrate is colorless, collect the filtrate, distill under reduced pressure at 45°C, extract 60% of the total weight of the organic solvent, and store the concentrated liquid at a temperature of -5°C Under certain conditions, let it stand for 20 hours, collect the precipitated solid, and dry it in vacuum to obtain 1.1 grams of lutein product with a purity of 91.7%.

example 2

[0034] Put 100 grams of marigold dried flower granules into the extraction tube (volume 150 ml) of the Soxhlet extractor, add 225 ml of petroleum ether with a boiling range of 30-60 ° C, 750 ml Ethanol water solution with a volume concentration of 90%, 0.3 g of potassium hydroxide, extraction-saponification for 8 hours at a heating temperature of 55°C, stirring, and dark conditions; after extraction-saponification, filter the extraction bottle of the Soxhlet extractor The mixture in the mixture, collect the filter residue, wash the filter residue with acetone until the filtrate is colorless, collect the filtrate, distill under reduced pressure at 30°C, extract 80% of the total weight of the organic solvent, and store the concentrated liquid at a temperature of -7°C Next, let it stand for 15 hours, collect the precipitated solid, and vacuum-dry to obtain 0.9 g of lutein product with a purity of 94.1%.

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Abstract

The invention belongs to the field of natural pigment preparation, and in particular relates to a preparation method of lutein. The present invention uses dried marigold flower granules as raw materials, comprising the following steps: (1) placing the dried marigold flower granules in the extraction tube of a Soxhlet extractor, adding petroleum ether and ethanol aqueous solution in sequence in the extraction bottle of the Soxhlet extractor , Potassium hydroxide, extraction-saponification is carried out under the conditions of heating, stirring and avoiding light. (2) Filter the mixture in the extraction bottle of the Soxhlet extractor, collect the filter residue, wash the filter residue with an organic solvent until the filtrate is colorless, collect the filtrate, concentrate under reduced pressure, and stand at low temperature to separate the concentrated solution to obtain the lutein product. The invention has the advantages of simplified process, simultaneous extraction of lutein ester and saponification of lutein ester, greatly shortening the preparation time of lutein, low energy consumption, little environmental pollution, conforming to environmental protection requirements, and suitable for industrial scale Production.

Description

technical field [0001] The invention belongs to the field of natural pigment preparation. Specifically relates to a preparation method of lutein. Background technique [0002] Lutein is a natural pigment with bright color, strong tinting power, safety and non-toxicity, and excellent antioxidant properties. It has been widely used in food, cosmetics, tobacco, livestock and poultry feed and other production fields. In recent years, scientific research has reported that lutein is related to reducing the risk of age-related macular degeneration (AMD) caused by oxidative damage to the macular area of ​​the retina, and lutein can effectively prevent arteriosclerosis, cataracts, and also inhibit the occurrence of cancer. A large amount of lutein exists in vegetables, flowers, fruits and some algae, especially in marigold and marigold. In plants, lutein is usually combined with fatty acids such as myristic acid, lauric acid and palmitic acid to form ester derivatives, but lutein e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24C09B61/00
Inventor 丁为民张也李俊雨
Owner HARBIN UNIV OF SCI & TECH