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A kind of acephate-degrading bacillus j1 and its application

A technology of acephate and bacillus, which is applied to bacteria, microorganisms, biochemical equipment and methods, etc., can solve problems such as pesticide residues and environmental pollution, and achieve the effect of high acephate degrading activity

Active Publication Date: 2016-06-01
JIANGSU SUGANG HESHUN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although acephate is less toxic than traditional organophosphorus pesticides such as methamidophos, due to its wide use, there are also problems of environmental pollution and pesticide residues

Method used

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  • A kind of acephate-degrading bacillus j1 and its application
  • A kind of acephate-degrading bacillus j1 and its application
  • A kind of acephate-degrading bacillus j1 and its application

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Embodiment Construction

[0018] In the following description, a lot of specific details are set forth in order to fully understand the present invention, but the present invention can also be implemented in other ways different from those described here, and those skilled in the art can do without departing from the connotation of the present invention. By analogy, the present invention is therefore not limited to the specific examples disclosed below.

[0019] 1. Main materials

[0020] (1) culture medium

[0021] Each 1L of 150mg / L, 300mg / L, 600mg / L, 1200mg / L acephate liquid medium composition: add acephate 150mg, 300mg, 600mg, 1200mg respectively, then add NaCl0.5g, KH 2 PO 4 0.5g, Na 2 HPO 4 1.5g, MgSO 4 0.5g, NH 4 NO 3 1g, FeCl 3 0.015g, CaCl 2 0.005g, prepared into solutions of different concentrations. Adjust the pH to 7.0 with HCl or NaOH. For the corresponding 1L solid medium, add 12g of agar powder to 1L liquid medium to prepare it.

[0022] (2) Instruments and equipment

[0023...

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Abstract

The invention discloses an acephate degrading bacillus J1 and application, and belongs to the field of biotechnology. The bacillus is preserved in the China General Microbiological Culture Collection Center with the preservation number CGMCC NO. 7793; the sequence of the bacillus is shown in SEQ ID NO.1. Experimental results show that the acephate degrading bacillus can survive in an environment where acephate is the unique carbon source, can grow well in 1200 mg / L acephate solid and fluid media. In a fluid medium of which the acephate content is 150 mg / L to 1200 mg / L, the acephate degradation rate of the bacillus J1 in 72 hours is more than 60 percent, that is, the acephate degradation activity of the bacillus J1 is very high. The acephate degrading bacillus can be widely applied to the degradation of acephate in a polluted environment, and has a good application value on the aspect of environmental remediation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an acephate-degrading bacillus J1 and its application. Background technique [0002] my country completely prohibits the use of highly toxic pesticides such as methamidophos, methyl parathion, and phosphamide in agricultural production. Due to its high efficiency and low toxicity, acephate has been widely used as a substitute for highly toxic pesticides in the control of vegetables, fruit trees and other crops, and its output is also increasing year by year. Although acephate is less toxic than traditional organophosphorus pesticides such as methamidophos, due to its wide use, there are also problems of environmental pollution and pesticide residues. Therefore, how to reduce the pollution and residue of acephate has become the focus of theoretical research and technology development. [0003] Microorganisms are an important group in the earth's ecological environment. Due to their wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/07A62D101/04A62D101/28
Inventor 侯进慧
Owner JIANGSU SUGANG HESHUN BIOTECH CO LTD