Wheatear in-vitro culture solution formula of hybrid induced haploid embryos of wheat and maize and preparation method thereof

An in vitro culture and haploid technology, which is applied in the field of agricultural biochemistry, can solve the problems of inconvenience of planting in the same season and inconvenience in research and improvement, and achieve the effects of high fruiting rate, full development and cost reduction of caryopsis.

Active Publication Date: 2014-05-14
FOOD CROPS RES INST YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In most parts of the world, wheat and corn cannot be planted in the same season. Wheat is planted in winter and spring, while corn is planted in spring and summer, which brings a lot of inconvenience to the further research and improvement of this technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Ear culture solution formula (P) of the present invention: 2,4-D100mg / L+ sucrose 35g / L+ potassium dihydrogen phosphate 2g / L+ sulfurous acid 6ml / L+ sodium tetraborate 0.5g / L+ carbonamide 2g / L+ silver nitrate 8mg / L.

[0025] Control ear culture medium formula (C): 2,4-D 100mg / L+ sucrose 40g / L+ silver nitrate 10mg / L+ sulfurous acid 8ml / L, the formula comes from the journal Euphytica (1998, 104: 17-23).

[0026] Preparation of culture solution: (1) Weigh 100mg of 2,4-dichlorophenoxyacetic acid into a beaker, add 50ml of sodium hydroxide with a concentration of 0.1N, and stir with a glass rod or stirrer to make 2,4-dichlorophenoxyacetic acid Phenoxyacetic acid is completely dissolved; (2) Add 600ml of tap water to a volumetric flask with a capacity of 1000ml, then add 35g of sucrose, 2g of potassium dihydrogen phosphate, 0.5g of sodium tetraborate, 2g of carbonamide, 6ml of sulfurous acid, and 8 mg of silver and dissolved 2,4-dichlorophenoxyacetic acid; (3) add tap water t...

Embodiment 2

[0036] Ear culture solution formula (P): 2,4-D100mg / L+ sucrose 45g / L+ potassium dihydrogen phosphate 4g / L+ sulfurous acid 8ml / L+ sodium tetraborate 1.5g / L+ carbonamide 4g / L+ silver nitrate 10mg / L .

[0037] Control (C) panicle culture solution formula: 2,4-D100mg / L+ sucrose 40g / L+ silver nitrate 10mg / L+ sulfurous acid 8ml / L+ potassium dihydrogen phosphate 3g / L, the formula is quoted from "Journal of Wheat Crops", 2008, 28(1):1-5.

[0038] The preparation method of two culture fluids is the same as embodiment 1.

[0039] In vitro culture of wheat-corn hybrid ears: In a comparative experiment on August 7, 2011, a total of 24 wheat ears of two wheat sterile lines K78S (S1) and K456S (S2) that had been pollinated with corn were cut from the field. Back in the room, 12 wheat ears of each wheat material were divided into two groups, with 6 ears in each group, inserted into the culture solution (P) prepared by this formula and the control culture solution (C) respectively and culti...

Embodiment 3

[0046] Ear culture medium formula (P): 2,4-D100mg / L+ sucrose 40g / L+ potassium dihydrogen phosphate 3g / L+ sulfurous acid 7ml / L, sodium tetraborate 1.0g / L, carbonamide 3g / L, silver nitrate 9mg / L. The preparation method of culture medium is the same as embodiment 1.

[0047] In vitro culture of wheat and corn hybrid wheat ears: On July 22, 2012, a total of 156 wheat ears from 15 wheat sterile line materials (S1-S15) that had been inoculated with corn pollen were cut from the field and inserted into the culture prepared by this formula. Cultured in liquid for 14 days (until August 5, 2012), the ear was taken, the caryopsis (grain) was peeled off, the embryo was peeled off under a dissecting microscope, and the number of haploid embryos of each sterile line material was counted. The test results are as follows:

[0048] S1: 12 spikes, 459 pollinated florets, 453 fruiting caryopsis, 180 haploid embryos, induction rate 39.2%

[0049] S2: 15 panicles, 611 pollinated florets, 574 fr...

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Abstract

The invention discloses a wheatear in-vitro culture solution formula of hybrid induced haploid embryos of wheat and maize and a preparation method thereof. The culture solution is prepared from the following compounds according to corresponding dosage: 100mg/L of 2,4-dichlorphenoxyacetic acid, 35-45g/L of cane sugar, 2-4g/L of monopotassium phosphate, 6-8ml/L of sulphurous acid, 0.5-1.5g/L of sodium tetraborate, 2-4g/L of carbamide, and 8-10mg/L of silver nitrate. The culture solution prepared by the formula is used for cultivating a wheatear which is pollinated by maize pollen and cut inside a room from a pan or a field, and inducing generation of haploid embryos. The inductivity of the haploid embryos after the formula is utilized can be improved by 10-15% in comparison with the existing wheatear culture solution under the condition of not changing all the other conditions.

Description

technical field [0001] The invention belongs to the technical field of agricultural biochemistry, and relates to a formula and a preparation method of a wheat ear in vitro culture solution for inducing haploid embryos by hybridization of wheat and corn. Background technique [0002] The corn pollen-induced wheat haploid technology established by Zenkteler (1984) and Laurie et al. (1986) has become one of the most efficient ways to produce wheat haploid through more than 20 years of research. Wheat haploid has important application value in screening gene mutants and gene function research, etc. Doubled haploid has important application value in gene mapping, construction of linkage genetic map, fixation of heterosis, and acceleration of wheat breeding process. [0003] Haploid embryo induction rate, also known as embryo formation rate and embryo yield rate, refers to the number of wheat haploid embryos that can be obtained per 100 emasculated wheat florets pollinated with co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨木军顾坚李绍祥刘琨李宏生田玉仙赵红杨和仙丁明亮
Owner FOOD CROPS RES INST YUNNAN ACAD OF AGRI SCI
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