Ultrasound micro-bubbles of targeted transfection peptide nucleic acid and preparation method and application thereof

Abstract

The invention relates to the field of genic ultrasound targeted transfection, in particular to an ultrasound micro-bubbles for targeted transfection of C-myc peptide nucleic acid and a preparation method thereof. The ultrasound micro-bubbles carries with the peptide nucleic acid, the peptide nucleic acid is prepared by antisense nucleic acid of C-myc proto-oncogene, and a nucleotide sequence of the antisense nucleic acid is 5'-AGCGAGGATATCTGG AAGAAATTCGAG-3'. The preparation method of the ultrasound micro-bubbles of the targeted transfection peptide nucleic acid comprises the following steps of adding the peptide nucleic acid prepared by the antisense nucleic acid of the C-myc proto-oncogene when the ultrasound micro-bubbles are prepared. A certain amount of PNA is added into an albumin solution when the ultrasound micro-bubbles are prepared, the PNA is wrapped by a micro-bubble wall albumin matrix and serves as a part of a micro-bubble structure, the targeted transfection is performed under the effect of an ultrasonic field, and the transfection efficiency is high and can reach up to more than 30%.

Description

technical field [0001] The invention relates to the field of gene ultrasonic targeted transfection, in particular to an ultrasonic microbubble for targeted transfection of peptide nucleic acid and its preparation method and application. Background technique [0002] C-myc proto-oncogene is one of the important members of the myc gene family, and it is an immediate early gene induced by a variety of growth factors. It is both a translocatable gene and a regulatable gene regulated by multiple substances. After C-Myc is synthesized in the cytoplasm, it forms oligomers with other proteins, then transfers to the nucleus, and binds to specific DNA sequences, thereby activating and inhibiting the transcription of many target genes, causing the growth and differentiation of cells. Change, play its role in physiological regulation and malignant transformation. It is a gene that can make cells proliferate indefinitely, acquire the function of immortalization, and promote cell divisi...

AI Technical Summary

Problems solved by technology

[0006] Although antisense PNA has the characteristics of strong specificity and good stability, and may inhibit or down-regulate the expression of target genes in the links of gene replication, transcription and translation, so as to achieve the purpose of treating diseases, the current problem is that it can be used as a drug. It is not easy to enter cells with PNA, and further increasing the uptake of antisense PNA by tissues and cells and the entry of antisense PNA into the nucleus have always been the key issues affecting its wide application in clinical practice.

In the past, when preparing ultrasonic microbubble gene carriers, most of the microbubbles were first prepared and then connected to genes or plasmids. It was difficult to estimate the combination stability and carrying capacity, and it was difficult to control the amount and effect of targeted transfection genes.

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