Agaricus bisporus strain sporocarp color SCAR-PCR identification method

A technology of Agaricus bisporus and identification method, which is applied in the directions of biochemical equipment and methods, and the determination/inspection of microorganisms, and can solve the problems of large workload and strong blindness of screening methods.

Active Publication Date: 2014-05-14
INST OF EDIBLE FUNGI FUJIAN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional screening methods are blind and need to be screened by fruiting. The workload is huge

Method used

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  • Agaricus bisporus strain sporocarp color SCAR-PCR identification method
  • Agaricus bisporus strain sporocarp color SCAR-PCR identification method

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Embodiment 1

[0017] 1. Taq DNA polymerase, PCR buffer, MgCl 2 , dNTPs were purchased from Xiamen Taijing Biological Co., Ltd., the DNA sequence was sequenced by Shanghai Sangon Co., Ltd., and the primers were synthesized by Shanghai Sangon Co., Ltd. The nucleotide sequence of the primers is:

[0018] SCAR4-F: 5'-CAAGTACTGAAAGTGTACTA-3',

[0019] SCAR4-R: 5'-GAGCCTTTCCATGTGCTCAC-3'.

[0020] 2. The 44 Agaricus bisporus strains are from the Genetics and Breeding Laboratory of the Edible Fungi Research Institute, Fujian Academy of Agricultural Sciences. See the strain number and strain name (Appendix: Table 1)

[0021] 3. DNA extraction and electrophoresis of Agaricus bisporus

[0022] DNA extraction and DNA electrophoresis of Agaricus bisporus were carried out according to literature [Chen Meiyuan, Liao Jianhua, Li Hongrong, Guo Zhongjie, Lu Zhenghui, Cai Danfeng, Wang Zesheng. DNA fingerprinting analysis of genetic diversity of Agaricus bisporus strains[J]. China Agricultural Science Bul...

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Abstract

The invention belongs to the field of edible mushroom molecular marking assistant breeding and particularly relates to an agaricus bisporus strain sporocarp color SCAR-PCR identification method. According to the method, SCAR molecular marking is used for carrying out PCR specific amplification on agaricus bisporus brown strains, and accordingly strain sporocarp color is identified. According to the method, fruiting tests are of no need, agaricus bisporus strain sporocarp color is specifically identified, consumed time is short, operation is easy, specificity is high, the products of 1273 bp are obtained by specific amplifying of the agaricus bisporus brown strains, strain sporocarp color can be quickly identified in agaricus bisporus crossbreeding, and the efficiency of agaricus bisporus crossbreeding is greatly improved.

Description

technical field [0001] The invention belongs to the field of molecular marker assisted breeding of edible fungi, and more specifically relates to a SCAR-PCR identification method for the color of the sporocarp of Agaricus bisporus strain. Background technique [0002] Agaricus bisporus ( Agaricus bisporus ) is the most widely cultivated edible fungus with the highest yield and largest consumption in the world, and has important economic value. At present, the main species of Agaricus bisporus in my country is single, and the main species As2796 has been used for 20 years since it was bred, and it is facing the inevitable degradation of commercial strains. On the other hand, due to the needs of the sustainable and healthy development of the industry and the increasingly diversified needs of production and the market, it is urgent to select and breed some special and excellent new strains suitable for different purposes. In recent years, the Institute of Edible Fungi of the ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/04C12Q1/686C12Q2531/113
Inventor 蔡志欣陈美元廖剑华李洪荣郭仲杰蔡丹凤王泽生
Owner INST OF EDIBLE FUNGI FUJIAN ACAD OF AGRI SCI
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