Application and use method of ligustilide in preparing skin-care product for preventing skin photoaging

A technology for skin photoaging and ligustilide, which is applied to skin care preparations, cosmetics, cosmetic preparations and other directions, can solve problems such as tumor occurrence, and achieve the effect of preventing skin photoaging

Active Publication Date: 2014-06-04
KARLIE COSMETICS MFG CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

EGFR is involved in regulating multiple biological processes such as cell proliferation, anti-apoptosis, migration and angiogenesis, and plays a very important role in mai...

Method used

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  • Application and use method of ligustilide in preparing skin-care product for preventing skin photoaging
  • Application and use method of ligustilide in preparing skin-care product for preventing skin photoaging
  • Application and use method of ligustilide in preparing skin-care product for preventing skin photoaging

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Ligustilide anti-light aging test

[0017] After resuscitating a purchased human keratinocyte, expand culture, grow for 3 generations, take half of the cells in a 6cm-diameter petri dish and spread them to a 24-well culture plate, and keep the remaining cells for cryopreservation. After the growth density of cells in the 24-well culture plate reached 60%, they were divided into negative control group, positive control group (all-trans retinoic acid) and experimental group (0.5% ligustilide solution). Add drugs to the experimental group and co-culture for 24 hours. After 24 hours, part of the control group and the experimental group sucked off the medium and added Trizol 500ul for total RNA extraction analysis, pipetted and transferred to a centrifuge tube and frozen to -20°C, and the other part of the control group and the experimental group , remove the medium, then add PBS, UVB ultraviolet treatment, the dose is 30mj / cm2, and finally restore the conditio...

Embodiment 2

[0022] Embodiment 2: Preparation of anti-photoaging emulsion

[0023] In the present embodiment, emulsion formula is as follows:

[0024] (A):

[0025]

[0026] (B):

[0027] Sodium Stearoyl Glutamate 0.20%

[0028] Deionized water to100%

[0029] (C):

[0030] Xanthan Gum 0.50%

[0031] Glycerin 6.00%

[0032] (D):

[0033] Bis(hydroxymethyl) imidazolidinyl urea 0.30%

[0034] Methylparaben 0.20%

[0035] Propylparaben 0.10%

[0036]Preparation method: Heat phases A and B to 80-85 degrees to dissolve completely, then add phase A to phase B for homogenization, and at the same time add phase C which is evenly mixed, homogenize for 5-10 minutes, after homogenization Stir in a water bath and keep warm for 30 minutes, then add other substances in phase D when the temperature drops below 50 degrees, stir evenly, and cool to room temperature.

Embodiment 3

[0037] Embodiment 3: Preparation of anti-photoaging moisturizing water

[0038] The recipe for this implementation is as follows:

[0039]

[0040] Preparation method: add the above-mentioned raw materials into sterilized deionized water in sequence, and stir while adding until it becomes transparent.

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Abstract

The invention discloses an application and use method of ligustilide in preparing a skin-care product for preventing skin photoaging. When the skin-care product is prepared, the ligustilide is taken as an active component and prepared into a ligustilide nano-liposome or nano-emulsion with the grain diameter less than 200 nanometers, and the mass percent concentration of the ligustilide nano-liposome or the nano-emulsion is 0.01%-10%. According to the application and use method disclosed by the invention, the influence of the ligustilide on the expression of a UVB (Ultraviolet B) induced skin keratinocyte photoaging key gene is inspected through a UVB induced skin keratinocyte model test, and a test result indicates that the skin-care product can effectively inhibit the degradation of collagen and the inflammatory reaction of cells and achieve the effect of preventing the skin photoaging; the ligustilide widely exists in angelica root volatile oil or cnidium officinale root volatile oil, and the ligustilide is directly added to the skin-care product for preventing the skin photoaging, or the angelica oil or cnidium officinale oil which contains the ligustilide is indirectly added to the skin-care product for preventing the skin photoaging.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application and application method of ligustilide in the preparation of skin care products for preventing skin photoaging. Background technique [0002] Skin photoaging is the premature aging of the skin caused by repeated exposure to the sun's ultraviolet rays. Acute exposure to UV rays from the sun can lead to sunburn (erythema), suppression of the immune system, DNA damage, and degradation of connective tissue, and long-term chronic UV exposure can cause photoaging of the skin, which can lead to skin cancer. With the deterioration of the environment and the reduction of the ozone layer, the amount of ultraviolet radiation irradiating the ground in the sun is gradually increasing, the phenomenon of photoaging of human skin is becoming more and more serious, and the incidence of skin cancer is increasing year by year, which seriously threatens the health of human skin...

Claims

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Application Information

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IPC IPC(8): A61K8/49A61Q19/08
Inventor 周华锋段明星姜梅高杨严泽民徐之伟
Owner KARLIE COSMETICS MFG CO LTD
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