Culture medium for inducing sweet potato cluster buds

Abstract

The invention relates to a culture medium for inducing sweet potato cluster buds, belonging to a sweet potato tissue culture technology. The culture medium is formed by dissolving kinetin (KT), 6-benzyl purine (6-BA), indoleacetic acid (IAA), casein hydrolysate (CH), cane sugar and agar into a basic culture medium (MS), wherein the dosage of all the components is as follows: 1L of MS, 1.0-2.0mg of KT, 1.0-2.0mg of 6-BA, 0.2-2.0mg of IAA, 200mg of CH, 20g of cane sugar and 6g of agar. The culture medium has the positive effects that after the culture medium is utilized, 8.6 buds can be induced by one stem apical meristem of a sweet potato, so that the induction efficiency is 860%, and the tissue culture efficiency is remarkably improved.

Description

technical field [0001] The invention relates to a medium for inducing clustered buds of sweet potato, which belongs to the technology of sweet potato tissue culture. In particular, a medium for inducing clustered shoots from the shoot apical meristem of sweet potato. Background technique [0002] The common method of culturing and propagating sweet potato shoot apical meristem is to induce differentiation of one shoot apical meristem into one bud, which is labor-intensive, time-consuming and inefficient. In the tissue culture and rapid propagation of many plants, the technology of inducing clustered buds is used to increase the reproduction coefficient and achieve twice the result with half the effort. Contents of the invention [0003] The purpose of the present invention is to overcome the problem of low reproduction coefficient in the tissue culture of sweet potato shoot tips, and provide a medium for efficiently inducing clustered buds of sweet potato, which is use...

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to overcome the problem of low reproductive coefficient in the shoot tip tissue culture of sweet potato...