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Method for detecting malto-oligosaccharide in beer through HPLC-ELSD (High-Performance Liquid Chromatography-Evaporative Light Scattering Detector)

A malto-oligosaccharide and beer technology is applied in the field of chemical analysis and detection to achieve the effects of good effect, rapid detection and high sensitivity

Inactive Publication Date: 2014-06-11
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on maltooligosaccharides is limited to the quantitative analysis of maltotriose and below, but there is no report on maltotetraose and above
And there is no effective detection method that can quantitatively analyze oligosaccharides such as maltose and maltotriose

Method used

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  • Method for detecting malto-oligosaccharide in beer through HPLC-ELSD (High-Performance Liquid Chromatography-Evaporative Light Scattering Detector)
  • Method for detecting malto-oligosaccharide in beer through HPLC-ELSD (High-Performance Liquid Chromatography-Evaporative Light Scattering Detector)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 Determination of maltooligosaccharide content in beer

[0021] (1) Sample preparation: Take 10.0 mL of commercially available beer, add the same volume of 10% (v / v) trifluoroacetic acid solution and place it at 4 °C for 1 hour, centrifuge at 8000 rpm for 10 min, take the supernatant and place it in another Put it in a new bottle and place it in an ultrasonic instrument for 60 minutes; filter it with a 0.45 μm organic filter membrane and a C18 extraction column, store it in a sample bottle, and prepare for sample injection.

[0022] (2) Set up the detection device:

[0023] Set the chromatographic conditions of the Shimadzu LC-20AB high-performance liquid chromatography workstation of the HPLC device: the specifications of the WatersXbridgeAmide column are: length 250 mm ╳ inner diameter 4.6 mm ╳ particle size 3.5 microns. Turn on the ELSD detector, set the temperature of the drift tube to 50°C, and the pressure of the carrier gas to 350KPa; after the set p...

Embodiment 2

[0028] Embodiment 2 method stability and reproducibility evaluation

[0029] Take a beer sample with known target content, add standard solutions of different mass concentrations, and prepare high (concentration of each maltooligosaccharide is 1000 μg / ml), medium (concentration of each maltooligosaccharide is 500 μg / ml) ), low (the concentration of each maltooligosaccharide is 10 micrograms / ml), and the mixed solution of three concentrations is measured 6 times according to the method of Example 1, and the RSD value is about 3%, indicating the stability and reproducibility of the method Good sex. The recovery rate takes the average value, the recovery rate of maltose is between 98.59%-109.09%; the recovery rate of maltotriose is 97.38%-108.47%; the recovery rate of maltotetraose is 101.64%-107.89%; The recovery rate of maltohexaose was 100.35%-106.85%; the recovery rate of maltohexaose was 98.31%-104.68%; content.

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Abstract

The invention discloses a method for detecting malto-oligosaccharide in beer through a HPLC-ELSD (High Performance Liquid Chromatography-Evaporative Light Scattering Detector) and belongs to the field of chemical analysis and detection. Through the method, a WatersXbridgeAmide column (250mm*4.6mm*3.5mm) is used; the ELSD is used; temperature of a drift tube is 50 DEG C; carrier gas pressure is 350KPa; a mobile phase A is an 80% acetonitrile water solution; a mobile phase B is 30% acetonitrile water solution; a flow rate is 1 milliliter per minute; a sampling feeding amount is 10 microliters; time is 30 minutes; the temperature of the column is 35 DEG C; the content of the malto-oligosaccharide in samples to be detected is calculated according to a standard curve. Through the method, the HPLC-ELSD is used, i.e., the high performance liquid chromatography and the evaporative light scattering detector are combined, so that quick and efficient detection is achieved; the method is not influenced by the mobile phases; meanwhile, the method is high in sensitivity, excellent in stability and reproducibility and simple, accurate and reliable to operate.

Description

technical field [0001] The invention relates to a method for measuring maltooligosaccharides in beer by using HPLC-ELSD, belonging to the field of chemical analysis and detection. Background technique [0002] Beer is rich in nutrients and is called "liquid bread". In fact, the most abundant content in beer is sugar, which not only provides most of the energy in beer, but also plays a role in the foam performance of beer. The free sugars in beer include monosaccharides, disaccharides and polysaccharides. The content of monosaccharides and high polysaccharides is relatively small, and the main component is oligosaccharides. How to quantitatively analyze maltooligosaccharides in beer has become a difficult problem in the study of its functional properties. [0003] Maltooligosaccharides include maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose and maltooctaose. At present, the research on maltooligosaccharides is limited to the quantitative an...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 李崎陈熙王金晶刘春凤李永仙郑飞云朱林江
Owner JIANGNAN UNIV
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