Application of diethylstilbestrol to preparation of medicine for treating or preventing influenza virus infection

A technology of diethylstilbestrol and influenza prevention, which is applied in the field of medicine and can solve the problems such as the related reports that the anti-influenza virus of diethylstilbestrol is not found.

Inactive Publication Date: 2014-06-25
武汉胜达康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] So far, no reports have been found on

Method used

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  • Application of diethylstilbestrol to preparation of medicine for treating or preventing influenza virus infection
  • Application of diethylstilbestrol to preparation of medicine for treating or preventing influenza virus infection
  • Application of diethylstilbestrol to preparation of medicine for treating or preventing influenza virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Evaluation of anti-influenza virus activity of diethylstilbestrol

[0026] 1. Experimental materials

[0027] 1.1 Cells, viruses and drugs

[0028]MDCK cells were purchased from ATCC, virus A / PuertoRico / 8 / 34 (H1N1) was amplified from chicken embryo culture, and diethylstilbestrol (CAS: 56-53-1) was purchased from Sigma.

[0029] 1.2 Experimental Instruments

[0030] Multifunctional detector PerkinElmer, inverted microscope Costar.

[0031] 2. Experimental methods and results

[0032] 2.1 Cell culture

[0033] 37°C, 5% CO 2 cultured in a humidified incubator. A DMEM medium containing 10% FBS, 100 U / mL of penicillin and streptomycin was used. Cells were subcultured after reaching 90% confluence, and the subculture ratio was 1 / 3–1 / 4.

[0034] 2.2 Virus culture

[0035] Take 9-11 day-old SPF chicken embryos, check them with an egg tester before inoculating the virus, and mark them at a position far away from the embryos, disinfect and punch holes, and in...

Embodiment 2

[0046] Example 2: Evaluation of broad-spectrum anti-influenza virus of diethylstilbestrol

[0047] On the basis of Example 1, this embodiment further detects that diethylstilbestrol is resistant to amantadine of influenza A virus subtype H1N1 strain, influenza A virus subtype H6N6 strain, influenza A virus subtype H7N8 strain and type B Antiviral activity of influenza virus.

[0048] 1. The virus strains used in the experiment include: A / WSN / S31N (H1N1), A / Duck / Hubei / 5 / 2010 (H6N6), A / Duck / Hubei / 216 / 1983 (H7N8), B / human / Huber / 1 / 2007

[0049] 2. Divide MDCK cells into 1.5×10 4 Cells / well were seeded in 96-well cell culture plates, cultured in a 37°C cell culture incubator for 14-18 hours, and the cells were grown into a single layer before use. Discard the medium in the well plate, wash twice with PBS, add 100TCID 50 A total of 200 μl of the virus liquid and drugs of various concentration gradients were cultured in a cell culture incubator at 37°C. The initial concentratio...

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PUM

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Abstract

The invention relates to the technical field of medicines and particularly discloses an application of diethylstilbestrol to preparation of a medicine for treating or preventing influenza virus infection. Firstly, the toxicity of diethylstilbestrol to an MDCK (madin darby canine kidney) cell is detected to obtain a result which shows that the maximum non-cytotoxic concentration of diethylstilbestrol is 25.0muM; secondly, the antiviral activity of diethylstilbestrol is detected within a complete non-cytotoxic concentration range to obtain a result which shows that the small-molecular compound has remarkable antiviral activity and the antiviral effect has a dose-dependent effect; finally, the antiviral activity of diethylstilbestrol to different types and subtypes of influenza viruses is detected to obtain a result which shows that diethylstilbestrol can inhibit the replication of all the detected influenza virus strains in a dose-dependent way, so that the anti-influenza virus activity of diethylstilbestrol is proved to have broad spectrum. Therefore, the invention firstly discloses diethylstilbestrol as a novel broad-spectrum anti-influenza virus medicine which can be used for preparing a medicine for preventing or treating influenza virus infection.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of diethylstilbestrol in the preparation of medicines for treating or preventing influenza virus infection. Background technique [0002] Influenza viruses belong to the family Orthomyxoviridae, the genus Influenzavirus. According to the differences in the antigenic and genetic characteristics of the virion nucleoprotein (NP) and matrix protein (M), influenza viruses are divided into three types: A, B, and C, also known as A, B, and C. The whole genome of influenza A virus consists of eight single-stranded negative-strand RNAs of different sizes, named after segment 1 to segment 8, respectively. The full-length viral genome is about 13.6kb, encoding 10 structural proteins (PB2, PB1, PA, HA, NP, NA, M1, M2, PB1-F2 and NS2 / NEP) and non-structural proteins (NS1). Influenza A viruses can be further divided into 17 H (H1-H17) and 10 N (N1-N10) subtypes according to ...

Claims

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Application Information

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IPC IPC(8): A61K31/05A61P31/16
Inventor 吴建国陈绪林邬开朗刘映乐潭秋萍刘芳
Owner 武汉胜达康生物科技有限公司
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