Application of diethylstilbestrol to preparation of medicine for treating or preventing influenza virus infection
A technology of diethylstilbestrol and influenza prevention, which is applied in the field of medicine and can solve the problems such as the related reports that the anti-influenza virus of diethylstilbestrol is not found.
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Embodiment 1
[0025] Example 1: Evaluation of anti-influenza virus activity of diethylstilbestrol
[0026] 1. Experimental materials
[0027] 1.1 Cells, viruses and drugs
[0028]MDCK cells were purchased from ATCC, virus A / PuertoRico / 8 / 34 (H1N1) was amplified from chicken embryo culture, and diethylstilbestrol (CAS: 56-53-1) was purchased from Sigma.
[0029] 1.2 Experimental Instruments
[0030] Multifunctional detector PerkinElmer, inverted microscope Costar.
[0031] 2. Experimental methods and results
[0032] 2.1 Cell culture
[0033] 37°C, 5% CO 2 cultured in a humidified incubator. A DMEM medium containing 10% FBS, 100 U / mL of penicillin and streptomycin was used. Cells were subcultured after reaching 90% confluence, and the subculture ratio was 1 / 3–1 / 4.
[0034] 2.2 Virus culture
[0035] Take 9-11 day-old SPF chicken embryos, check them with an egg tester before inoculating the virus, and mark them at a position far away from the embryos, disinfect and punch holes, and in...
Embodiment 2
[0046] Example 2: Evaluation of broad-spectrum anti-influenza virus of diethylstilbestrol
[0047] On the basis of Example 1, this embodiment further detects that diethylstilbestrol is resistant to amantadine of influenza A virus subtype H1N1 strain, influenza A virus subtype H6N6 strain, influenza A virus subtype H7N8 strain and type B Antiviral activity of influenza virus.
[0048] 1. The virus strains used in the experiment include: A / WSN / S31N (H1N1), A / Duck / Hubei / 5 / 2010 (H6N6), A / Duck / Hubei / 216 / 1983 (H7N8), B / human / Huber / 1 / 2007
[0049] 2. Divide MDCK cells into 1.5×10 4 Cells / well were seeded in 96-well cell culture plates, cultured in a 37°C cell culture incubator for 14-18 hours, and the cells were grown into a single layer before use. Discard the medium in the well plate, wash twice with PBS, add 100TCID 50 A total of 200 μl of the virus liquid and drugs of various concentration gradients were cultured in a cell culture incubator at 37°C. The initial concentratio...
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